Rpmi 1640 11875119

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

RPMI 1640 (11875119) is a commonly used cell culture medium formulated to support the growth of a wide variety of cell types. It provides a defined and balanced combination of nutrients, amino acids, and vitamins required for cell proliferation and maintenance in vitro.

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Lab products found in correlation

Naf s6776, by Merck Group (1 mentions) Lymphocyte separation medium dkw33 r0100, by Dakewe (1 mentions) Fetal bovine serum 16000044, by Thermo Fisher Scientific (1 mentions) Rabbit igg 7074p2, by Cell Signaling Technology (1 mentions) Mouse igg 7076p2, by Cell Signaling Technology (1 mentions) Nicl2 6h2o n6136, by Merck Group (1 mentions)

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RPMI 1640 (15891 citations)

2 protocols using rpmi 1640 11875119

Apoptosis Pathway Regulation in Lymphocytes

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NaF (S6776) was purchased from Sigma Aldrich, UK. Lymphocyte separation medium (DKW33-R0100) were supplied by Dakewe Biotech Company, China. JC-1 mitochondrial membrane potential assay kit (C2006), ROS Assay kit (S0033), RIPA lysis buffer (P0013C), BCA Protein Assay Kit (P0012), Cell Counting Kit-8 (CCK-8) (C0038) and Hoechst 33258 (C1011) were obtained from Beyotime Biotechnology, China. RPMI 1640 (11875119) and fetal bovine serum (16000044) were supplied by Gibco, UK. The mouse Bc1-2 (3498T), Bcl-xl (2764T), Bax (14796S), Bak (12105T), caspase-9 (9508T), cleaved caspase-8 (8592T), cleaved caspase-7 (8438S), cleaved caspase-6 (9761T), cleaved caspase-3 (9664T) antibodies, rabbit IgG (7074P2) and mouse IgG (7076P2) were obtained from Cell Signaling Technology, UK. Fas (ab82419) and FasL (ab16104) were purchased from Abcam, UK. All other reagents used were analytical grade.

Deng H., Kuang P., Cui H., Chen L., Fang J., Zuo Z., Deng J., Wang X, & Zhao L. (2016). Sodium fluoride induces apoptosis in cultured splenic lymphocytes from mice. Oncotarget, 7(42), 67880-67900.

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Cobalt and Nickel Chloride Cell Culturing

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NiCl₂×6H₂O (N6136) and CoCl₂×6H₂O (C8661) were purchased from Sigma. RPMI-1640 (11875119) and DMEM (12430062) media were Gibco. F12K (30-2004) medium was purchased from ATCC. Fetal bovine serum (FBS-BBT-5XM) was acquired from RMBIO.

Luczak M.W, & Zhitkovich A. (2017). Nickel-induced HIF-1α promotes growth arrest and senescence in normal human cells but lacks toxic effects in transformed cells. Toxicology and applied pharmacology, 331, 94-100.

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