Alexa 488 conjugated donkey anti rat

Manufactured by Jackson ImmunoResearch

Sourced in United States

Alexa Fluor® 488-conjugated donkey anti-rat is a secondary antibody reagent used for the detection and visualization of rat primary antibodies in various immunoassay applications. The Alexa Fluor® 488 dye provides bright, photostable green fluorescence.

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Lab products found in correlation

Lsm 700, by Zeiss (2 mentions) Alexa 488 conjugated donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) Anti collagen 1, by Abcam (1 mentions) Bisbenzimide h 33258, by Merck Group (1 mentions) Anti il 33, by R&D Systems (1 mentions) Lsm image browser, by Zeiss (1 mentions) Cy5 conjugated anti rat, by Jackson ImmunoResearch (1 mentions) Rat anti gfp, by Nacalai Tesque (1 mentions) Cy3 conjugated donkey anti mouse, by Jackson ImmunoResearch (1 mentions) Rabbit anti gm130, by Abcam (1 mentions) Cy5 conjugated anti guinea pig, by Jackson ImmunoResearch (1 mentions) Rabbit anti gfp, by Nacalai Tesque (1 mentions) Cy5 conjugated anti rabbit, by Jackson ImmunoResearch (1 mentions) Lsm 810, by Zeiss (1 mentions) Alexa 488 conjugated donkey anti rabbit, by Jackson ImmunoResearch (1 mentions)

2 protocols using alexa 488 conjugated donkey anti rat

Immunological Analysis of Mouse IL-5 Effects

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For drug administration, recombinant Mouse IL-5, rat anti-mouse IL-5 (TRFK5) and Rat IgG1 κ isotype control (R3–34) were purchased from BD Biosciences (San Diego, CA). The panel of antibodies used in flow cytometry are listed in Supplementary table 1. For immunofluorescence staining, the following primary antibodies were used: anti-IL33 (5 μg mL⁻¹ + 0.01% Triton X-100 in PBB, goat IgG; R&D Systems, Minneapolis, MN), anti-collagen I (2 μg mL⁻¹ in PBB, rabbit IgG; Abcam, Cambridge, MA); anti-CD45 polyclonal antibody (2 μg mL⁻¹ + 0.01% Triton X-100 in PBB, rat IgG; Abcam). Alexa Cy5-conjugated donkey anti-goat IgG (Jackson ImmunoResearch, West Grove, PA), Alexa 488-conjugated donkey anti-rat (Jackson ImmunoResearch) and Alexa 488-conjugated donkey anti-rabbit (Molecular Probes, San Jose, CA) were used as secondary antibodies. Nuclear staining was performed with Bisbenzimide H 33258 (Sigma-Aldrich, St Louis, MO).

Xu H., Xu J., Xu L., Jin S., Turnquist H.R., Hoffman R., Loughran P., Billiar T.R, & Deng M. (2018). Interleukin-33 contributes to ILC2 activation and early inflammation-associated lung injury during abdominal sepsis. Immunology and cell biology, 96(9), 935-947.

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Immunostaining Embryos for Confocal Imaging

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Embryos were stained with antibodies as previously described [37 (link)] and observed with a Zeiss LSM 700 or LSM 810 confocal laser microscope, and the results were analyzed using the LSM image browser (Zeiss, Jena, Germany) and ImageJ software (Version 13.0.6, NIH, Bethesda, MD, USA) [38 (link)]. We used the following primary antibodies: rat anti-Elav (7E8A10, 1:500) [39 (link)], mouse anti-NICD (C17.9C6, 1:250) [40 (link)], mouse anti-Crumbs (Cq4, 1:250) [41 (link)], rat anti-E-Cadherin (DCAD2 1:500) [42 (link)], guinea pig anti-FL-Hrs (GP30, 1:1000) [43 (link)], rabbit anti-Rab7 (1:5000) [44 (link)], rabbit anti-Rab11 (1:4000) [44 (link)], rabbit anti-GM130 (1:50, Abcam, Cambridge, MA, USA) [45 (link)], rabbit anti-GFP (1:250, 598 MBL) [46 (link)], and rat anti-GFP (1:250, Nacalai Tesque, Kyoto, Japan).
We used the following secondary antibodies: Cy3-conjugated donkey anti-mouse, Cy5-conjugated anti-rabbit, Cy-5 conjugated anti-rat, Cy5-conjugated anti-guinea pig, Alexa488-conjugated donkey anti-rat, and Alexa488-conjugated donkey anti-rabbit (all from Jackson Immunoresearch, West Grove, PA, USA).

Nurmahdi H., Hasegawa M., Mujizah E.Y., Sasamura T., Inaki M., Yamamoto S., Yamakawa T, & Matsuno K. (2022). Notch Missense Mutations in Drosophila Reveal Functions of Specific EGF-like Repeats in Notch Folding, Trafficking, and Signaling. Biomolecules, 12(12), 1752.

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