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Fgfr3

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FGFR3 is a laboratory equipment product offered by Thermo Fisher Scientific. It is a receptor tyrosine kinase that plays a role in the regulation of cell growth, differentiation, and angiogenesis. The core function of FGFR3 is to serve as a tool for researchers to study various biological processes and pathways involving this receptor.

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Lab products found in correlation

Fgfr2, by Thermo Fisher Scientific (2 mentions) M mlv reverse transcriptase, by Thermo Fisher Scientific (2 mentions) Bone morphogenetic protein 4 (bmp4), by Thermo Fisher Scientific (1 mentions) Ve cadherin, by Thermo Fisher Scientific (1 mentions) Maxwell rsc mirna tissue kit, by Promega (1 mentions) Nanog, by Thermo Fisher Scientific (1 mentions) Vegf a, by Thermo Fisher Scientific (1 mentions) Snai1, by Thermo Fisher Scientific (1 mentions) β catenin, by Thermo Fisher Scientific (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Taqman probe, by Thermo Fisher Scientific (1 mentions) Maxwell rsc instrument, by Promega (1 mentions) β klotho, by Thermo Fisher Scientific (1 mentions) β actin, by Thermo Fisher Scientific (1 mentions) Fgfr1, by Thermo Fisher Scientific (1 mentions) Innuprep rna mini kit, by Analytik Jena (1 mentions) Taqman assay, by Thermo Fisher Scientific (1 mentions) Dasatinib, by Selleck Chemicals (1 mentions) Erlotinib, by Selleck Chemicals (1 mentions) α ketoglutaric acid sodium salt, by Merck Group (1 mentions)

5 protocols using fgfr3

1

Comprehensive Transcriptional Profiling of Angiogenic Pathways

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Total RNA was extracted with Maxwell RSC miRNA Tissue Kit and processed with Maxwell RSC Instrument (Promega). RNAs were quantified with DeNovix spectrophotometer (DeNovix, Wilmington) and reverse transcribed using High-Capacity cDNA reverse transcription kit (Applied Biosystems, Thermofisher Scientific). cDNAs were used to run a real-time PCR using TaqMan probes specific for the following genes: VE-cadherin, ICAM2, TIE1, TEK, FLK1, TGFβ1, TGFBR1, TGFBR2, ACTA2, CTGF, TBX3, SPP1, ACE, Sema7A, Nanog, BMP4, FGFR3, HES5, T, VEGFA, SNAI1, SNAI2, FGFR2, WNT3, WNT4, WNT6, WNT10A, FZD4, FZD6, LRP5, LRP6, β-catenin, TBP (Applied Biosystems. Thermofisher Scientific). Expression data were analyzed with SDS 2.3 software.
Gualandris A., Noghero A., Cora’ D., Astanina E., Arese M, & Bussolino F. (2021). Role of TGFβ1 and WNT6 in FGF2 and BMP4-driven endothelial differentiation of murine embryonic stem cells. Angiogenesis, 25(1), 113-128.
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2

Quantification of mRNA Levels in Mice

Cited 1 time
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The mRNA levels of TNF-α, IL-6, IL-1β, collagen-1, FGFR1, β-Klotho, FGFR2, FGFR3, and FGFR4 were quantified by qRT-PCR as described previously.47 (link), 48 (link) The primers of TNF-α (Mm00443285_m1), IL-6 (Mm00446190_m1), IL-1β (Mm00434228_m1), collagen-1 (Mm01302043), FGFR1 (Mm00438930_m1), β-Klotho (Mm00473122_m1), FGFR2 (Mm01269930_m1), FGFR3 (Mm00433294_m1), FGFR4 (Mm01341852_m1), and β-actin (Mm00607939) were from Applied Biosystems (Carlsbad, CA, USA). The expression levels of the target genes were normalized to that of the housekeeping gene β-actin.
Wang S., Wang Y., Zhang Z., Liu Q, & Gu J. (2017). Cardioprotective effects of fibroblast growth factor 21 against doxorubicin-induced toxicity via the SIRT1/LKB1/AMPK pathway. Cell Death & Disease, 8(8), e3018-.
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3

Immunohistochemical Analysis of FGFR

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Paraffin-embedded tissue sections were used for immunochemical staining. Antibodies for immunochemical staining were as follows: FGFR1 (PA5-25979, Thermo Fisher Scientific), FGFR2 (PA1-24763, Thermo Fisher Scientific), FGFR3 (MA5-32620, Thermo Fisher Scientific), FGFR4 (PA5-119550, Thermo Fisher Scientific).
Wang C., Cao Q., Zhang S., Liu H., Duan H., Xia W., Shen H, & Wang C. (2023). Anlotinib Enhances the Therapeutic Effect of Bladder Cancer with GSDMB Expression: Analyzed from TCGA Bladder Cancer Database & Mouse Bladder Cancer Cell Line. Pharmacogenomics and Personalized Medicine, 16, 219-228.
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4

Quantitative Expression Analysis of FGF Receptors

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Cells were grown in 25 cm2 flasks to about 80% confluence and total RNA was extracted with the innuPREP RNA mini kit (Analytik Jena, Jena, Germany) according to the manufacturer´s instructions. RNA was reverse transcribed with M-MLV reverse transcriptase (Thermo Fisher Scientific) and the resulting cDNAs were used as templates for qRT-PCR analysis with Taqman assays (FGFR1: Hs00913142m1, FGFR2: Hs01552918m1, FGFR3: Hs00179829m1, FGFR4: Hs01106908m1, all from Thermo Fisher Scientific). Relative transcript levels were calculated as 2−ΔCt × 105 normalized to the two house-keeping genes GAPDH (Hs99999905m1) and beta-actin (Hs99999903m1).
Vlacic G., Hoda M.A., Klikovits T., Sinn K., Gschwandtner E., Mohorcic K., Schelch K., Pirker C., Peter-Vörösmarty B., Brankovic J., Dome B., Laszlo V., Cufer T., Rozman A., Klepetko W., Grasl-Kraupp B., Hegedus B., Berger W., Kern I, & Grusch M. (2019). Expression of FGFR1–4 in Malignant Pleural Mesothelioma Tissue and Corresponding Cell Lines and its Relationship to Patient Survival and FGFR Inhibitor Sensitivity. Cells, 8(9), 1091.
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5

Kinase Activity Assay Protocol

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Myelin basic protein from bovine, DL-isocitric acid trisodium salt hydrate, β-nicotinamide adenine dinucleotide phosphate hydrate (NADP+), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH), α-ketoglutaric acid sodium salt, diaphorase from Clostridium kluyveri, resazurin sodium salt, and ATP disodium salt hydrate were purchased from Sigma-Aldrich. Ketoglutaric acid sodium salt, α-[1-14C], 50μCi (1.85MBq) and glucose, D-[U-14C]- were from PerkinElmer. Glutamine L-[5-14C] and isocitric acid[3H(G)] were from ARC. Inhibitors including dovitinib (TKI-258, CHIR-258), imatinib, AG-490, quizartinib, PP2, erlotinib, ruxolitinib, dasatinib and AG-120 were purchased from Selleckchem. Recombinant proteins including FLT3, SRC, PDGFRα, PDGFRβ, EGFR, MET, KIT, JAK2, FGFR3 and ABL1 were purchased from Thermo Fisher.
Chen D., Xia S., Wang M., Lin R., Li Y., Mao H., Aguiar M., Famulare C.A., Shih A.H., Brennan C.W., Gao X., Pan Y., Liu S., Fan J., Jin L., Song L., Zhou A., Mukherjee J., Pieper R.O., Mishra A., Peng J., Arellano M., Blum W.G., Lonial S., Boggon T.J., Levine R.L, & Chen J. (2019). Mutant and wild-type isocitrate dehydrogenase 1 share enhancing mechanisms involving distinct tyrosine kinase cascades in cancer. Cancer discovery, 9(6), 756-777.
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