Spleen, lung, and MLN samples were aseptically removed from mice at various time-points following influenza virus infection. Lungs were digested with collagenase (Sigma), whilst spleens and MLNs were disrupted with glass microscope slides to generate single-cell suspensions. Spleen cell suspensions were enriched for T cells following B cell panning on plates coated with goat anti-mouse IgG and IgM antibody (Jackson ImmunoResearch, West Grove, PA) for 1 h at 37°C.
Goat anti mouse igg and igm antibody
Manufactured by Jackson ImmunoResearch
Goat anti-mouse IgG and IgM antibody is a laboratory reagent used in immunoassays and other immunological applications. It is an antibody produced in goats that specifically binds to mouse immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies.
Automatically generated - may contain errors
2 protocols using goat anti mouse igg and igm antibody
1
Influenza Infection Immune Response
2
Western Blot Analysis of PARP and TRPV1
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Cells were collected and then boiled for 5 min at 100 °C in a reducing sample buffer containing 2-mercaptoethanol. Then, the samples were separated by 10% (w/v) SDS-PAGE, and the separated proteins were transferred to polyvinylidene difluoride membrane. Membranes were blocked with 5% (w/v) skim milk in Tris-buffered saline and then incubated with an anti-PARP (poly (ADP-ribose) polymerase) antibody (Roche Diagnostics), TRPV1 rabbit polyclonal antibody, α-tubulin monoclonal antibody (Sigma) or rabbit IgG as a control (Sigma) for 1 h at room temperature, followed by a HRP-conjugated goat anti-rabbit IgG (KLP) or goat anti-mouse IgG and IgM antibody (Jackson ImmunoResearch) for 1 h at room temperature. Labeled proteins were visualized with Pierce ECL western blotting substrate, according to the manufacturer's protocol (Thermo Fisher Scientific).
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