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Discover ogr 500 kit

Manufactured by DNA Genotek
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The Discover OGR-500 kit is a laboratory equipment product offered by DNA Genotek. It serves as a core function for sample collection and preparation. The kit provides the necessary components for obtaining high-quality DNA samples from various sources.

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Lab products found in correlation

Qiaamp dna investigator kit, by Qiagen (2 mentions) Oracollect oc 175 kit, by DNA Genotek (2 mentions) Quick dna urine kit, by Zymo Research (2 mentions) Gentra puregene blood kit, by Qiagen (2 mentions) Prepit l2p, by DNA Genotek (2 mentions)

3 protocols using discover ogr 500 kit

1

Genomic DNA Extraction from Diverse Biospecimens

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Initial ES in the BHCMG and BG cohorts was performed on the blood samples in greater than 95% of cases. In the remainder of cases, it was saliva. For validation experiments, peripheral blood DNA was extracted using the Gentra Puregene Blood kit (Qiagen, Germantown, MD, USA). For the selected cases from the BG cohort, at least five hairs with follicles were collected, and DNA was extracted using the QIAamp DNA Investigator Kit (Qiagen). Saliva was collected using the ORAgene Discover OGR-500 kit (DNA Genotek, Ottawa, Canada). Buccal cells were collected using the ORAcollect OC-175 kit (DNA Genotek). Both saliva and buccal cell DNA were extracted using the prepIT-L2P (DNA Genotek). DNA from urine was extracted using the Quick-DNA Urine Kit (Zymo Research, Irvine, CA, USA). All procedures followed the manufacturer’s instructions.
Gambin T., Liu Q., Karolak J.A., Grochowski C.M., Xie N.G., Wu L.R., Yan Y.H., Cao Y., Akdemir Z.H., Wilson T.A., Jhangiani S.N., Chen E., Eng C.M., Muzny D., Posey J.E., Yang Y., Zhang D.Y., Shaw C., Liu P., Lupski J.R, & Stankiewicz P. (2020). Low-level parental somatic mosaic SNVs in exomes from a large cohort of trios with diverse suspected Mendelian conditions. Genetics in medicine : official journal of the American College of Medical Genetics, 22(11), 1768-1776.
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2

Genomic DNA Extraction from Diverse Biospecimens

Check if the same lab product or an alternative is used in the 5 most similar protocols
Initial ES in the BHCMG and BG cohorts was performed on the blood samples in greater than 95% of cases. In the remainder of cases, it was saliva. For validation experiments, peripheral blood DNA was extracted using the Gentra Puregene Blood kit (Qiagen, Germantown, MD, USA). For the selected cases from the BG cohort, at least five hairs with follicles were collected, and DNA was extracted using the QIAamp DNA Investigator Kit (Qiagen). Saliva was collected using the ORAgene Discover OGR-500 kit (DNA Genotek, Ottawa, Canada). Buccal cells were collected using the ORAcollect OC-175 kit (DNA Genotek). Both saliva and buccal cell DNA were extracted using the prepIT-L2P (DNA Genotek). DNA from urine was extracted using the Quick-DNA Urine Kit (Zymo Research, Irvine, CA, USA). All procedures followed the manufacturer’s instructions.
Gambin T., Liu Q., Karolak J.A., Grochowski C.M., Xie N.G., Wu L.R., Yan Y.H., Cao Y., Akdemir Z.H., Wilson T.A., Jhangiani S.N., Chen E., Eng C.M., Muzny D., Posey J.E., Yang Y., Zhang D.Y., Shaw C., Liu P., Lupski J.R, & Stankiewicz P. (2020). Low-level parental somatic mosaic SNVs in exomes from a large cohort of trios with diverse suspected Mendelian conditions. Genetics in medicine : official journal of the American College of Medical Genetics, 22(11), 1768-1776.
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3

Genotyping for ApoE4 Allele in AD

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Apolipoprotein E ɛ4 (ApoE4) is the most common risk gene for AD [13 (link)] and we anticipated that the at-risk group would have more carriers of ApoE4 than the control group. Subjects were genotyped for carrier status of ApoE (2, 3, 4). Buccal cells were collected from each subject (Oragene Discover OGR-500 kit, DNA Genotek Inc., Ottowa, ON, Canada) and DNA was isolated and genotyped by Vanderbilt Technologies for Advanced Genomics Subjects using Applied Biosystems Taqman 7900 HT Instrument (Life Technologies, Grand Island, NY, USA) with primers to rs7412 and rs429358.
Mason E.J., Hussey E.P., Molitor R.J., Ko P.C., Donahue M.J, & Ally B.A. (2017). Family History of Alzheimer’s Disease is Associated with Impaired Perceptual Discrimination of Novel Objects. Journal of Alzheimer's Disease, 57(3), 735-745.
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