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2 protocols using zn 2 chloride

1

Comprehensive Biochemical Reagents Protocol

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HEPES (Pharmagrade), Tricine (Pharmagrade), La(III)chloride heptahydrate (99.999%), TEA chloride, Chelex 100 (sodium form), Zn(II)chloride (99.999%), pyruvic acid (98%), ATP, oxaloacetic acid, FBS, hygromycin B, geneticin, penicillin-streptomycin, hydrochloric acid (TraceSelect), and 70% nitric acid (ACS reagent) were purchased from Sigma-Aldrich. Anhydrous ethanol, diethylpyrocarbonate (high purity), sodium hydroxide 30% solution (suprapur), sodium chloride (puratronic, 99.999%; AlfaAesar), potassium chloride (puratronic, 99.997%; AlfaAesar) cesium chloride (99.999%; AlfaAesar), calcium chloride (99.99%; AlfaAesar), 50% cesium hydroxide solution (99.9%; AlfaAesar), D-glucose (AnalaR Normapur), and potassium hydroxide (99.98%) were purchased from VWR. 1,000 mM stock solutions of diethyl pyrocarbonate (DEPC) in anhydrous ethanol were prepared on the day of the experiments, kept on ice, and diluted into standard external solution immediately before use.
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2

Synthesis of Magnetic Nanoparticles

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All solvents were of analytical grade and used with no modifications. Fe(III) chloride hexahydrate, Fe(II) chloride tetrahydrate, Mn(II) chloride tetrahydrate, Zn(II) chloride, trisodium citrate dihydrate, ammonium hydroxide and epoxy resin kit were purchased from Sigma-Aldrich. Dextran (70 kDa) was purchased from Carl Roth. Ultrapure water produced with a
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