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Celite

Manufactured by Teledyne
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Celite is a diatomaceous earth-based filtration aid used in various laboratory applications. It is a porous, inert material that provides a high surface area for efficient filtration of liquids and particulates. Celite can be used to facilitate the separation and purification of a wide range of substances, such as chemicals, pharmaceuticals, and biological samples.

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Lab products found in correlation

25 gram solid phase cartridge, by Teledyne (2 mentions) Celite 545, by Thermo Fisher Scientific (2 mentions)

2 protocols using celite

1

Large-scale extraction and purification of fungal compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
A. fumigatus strains were inoculated (1.0×106 spores mL−1) into 1 L GMM43 (link) in a 2 L Erlenmeyer flask at 37 °C with shaking at 220 rpm. After 3.5 days, liquid fungal cultures including fungal tissue and media were frozen using a dry ice acetone bath, and lyophilized. The lyophilized residues were extracted with 500 mL of MeOH for 1 h with vigorous stirring. Extracts were filtered over cotton and evaporated on Celite-545 (Acros Organics) to dryness. The dry Celite was then loaded into a 25 gram solid phase cartridge (Isco) and subjected to large-scale reverse phase chromatography (see Chromatographic enrichment section below for details). Compounds 4 and 5 are also minor components of the culture media.
Baccile J.A., Spraker J.E., Le H.H., Brandenburger E., Gomez C., Bok J.W., Macheleidt J., Brakhage A.A., Hoffmeister D., Keller N.P, & Schroeder F.C. (2016). Plant-like biosynthesis of isoquinoline alkaloids in Aspergillus fumigatus. Nature chemical biology, 12(6), 419-424.
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2

Large-scale extraction and purification of fungal compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
A. fumigatus strains were inoculated (1.0×106 spores mL−1) into 1 L GMM43 (link) in a 2 L Erlenmeyer flask at 37 °C with shaking at 220 rpm. After 3.5 days, liquid fungal cultures including fungal tissue and media were frozen using a dry ice acetone bath, and lyophilized. The lyophilized residues were extracted with 500 mL of MeOH for 1 h with vigorous stirring. Extracts were filtered over cotton and evaporated on Celite-545 (Acros Organics) to dryness. The dry Celite was then loaded into a 25 gram solid phase cartridge (Isco) and subjected to large-scale reverse phase chromatography (see Chromatographic enrichment section below for details). Compounds 4 and 5 are also minor components of the culture media.
Baccile J.A., Spraker J.E., Le H.H., Brandenburger E., Gomez C., Bok J.W., Macheleidt J., Brakhage A.A., Hoffmeister D., Keller N.P, & Schroeder F.C. (2016). Plant-like biosynthesis of isoquinoline alkaloids in Aspergillus fumigatus. Nature chemical biology, 12(6), 419-424.
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