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2 protocols using tsa t1952

1

Antibody Panel for Cell Analysis

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The following antibodies were used in this research: JMJD5 (ab106391) from Abcam; β-actin (sc-47778), BrdU (sc-32323), P53 (sc-6243), CDKN1A (sc53870) and Flag (sc-166355) from Santa Cruz Biotechnology; and histone modification-specific antibodies were purchased from Abcam and Millipore. Propidium iodide (P4864), bromodeoxyuridine (B9258), DAC (A3656) and TSA (T1952) were obtained from Sigma.
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2

Evaluating Sirtuin-Mediated β-Catenin Regulation

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The following primary antibodies were commercially obtained: anti-acetylated-lysine antibody (Cell Signaling Technology, 9441); anti-SIRT4 antibody (Abcam, ab10140); anti-β-catenin antibody (Cell Signaling Technology, 9562); anti-HA antibody (Santa Cruz, sc-7392); anti-Myc antibody (Santa Cruz, sc-40); anti-Flag antibody (Sigma, F1804); anti-tubulin antibody (Sigma, T619); anti-phospho-S33/37/T41 β-catenin antibody (Cell Signaling Technology, 9561); anti-β-TrCP antibody (Cell Signaling Technology, 4394); anti-β-TrCP antibody (Cell Signaling Technology, 11984) and anti-dishevelled 2 antibody (Abcam, ab228804); anti-GSK3β antibody (Cell Signaling Technology, 12456); anti-APC antibody (Abcam, ab40778). FLAG peptide was purchased from Sigma–Aldrich (St. Louis, MO). Small interfering RNAs (siRNAs) against SIRT family mRNAs were purchased from Santa Cruz Biotechnology, Inc. (SIRT1 siRNA (h), sc-40986, SIRT3 siRNA (h), sc-61555, SIRT4 siRNA (h), sc-63024, SIRT5 siRNA (h), sc-63026, SIRT6 siRNA (h), sc-63028, SIRT7 siRNA (h), sc-63030). Chemicals, including NAM (A2984) and TSA (T1952), were obtained from Sigma. Wnt3a-conditioned medium was derived from stably transfected L-Wnt3a cells.
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