Electronic circular dichroism and optical rotation analyses were made using a Shimadzu HPLC chromatograph coupled to a JASCO CD-2095plus CD detector and a PDR advanced laser polarimeter detector, and data were acquired using JASCO ChromNAV software version 2.01.05. Chiroptical measurements were obtained through a chromatographic analysis using a Phenomenex Lux Cellulose-1 column (150 mm x 4.5 mm, 5 µm) with a mobile phase composed of n-hexane/isopropanol 9:1 at a flow rate of 1.0 ml/min. Optical rotation response was measured at 670 nm, while UV and ECD spectra were measured between 220 and 420 nm by stop-flow while each compound was in the CD detector cell. Although this HPLC-CD-OR measurement methodology was not used for the quantitative analyses of the pure isolated flavanones, a validation procedure was performed using pure racemic flavanone and obtaining consistent results with those described in a previous work (Muñoz et al., 2013 (link)).
Hplc chromatograph
Manufactured by Jasco
Sourced in Japan
The HPLC chromatograph is a laboratory instrument used for the separation, identification, and quantification of chemical compounds in a mixture. It utilizes high-pressure liquid chromatography to achieve the separation process.
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Lab products found in correlation
2 protocols using hplc chromatograph
1
Chiroptical Analysis of Flavanones
2
Tocol Analysis by HPLC Fluorescence
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Tocols were evaluated following the international standard ISO 9936 ( 2006), with some modifications as described by Casal et al. (2010) . Briefly, tocochromanols were separated on a HPLC chromatograph (Jasco, Tokyo, Japan) equipped with a pump (PU-980 model), mixing chamber (HG 980-30) and an autosampler (AS2057 Plus model).
The detection was performed by the fluorescence detector FP2020 Plus model at 290 nm (excitation) and 330 nm (emission) wavelengths. The tocopherols and tocotrienols separation was performed on a normal phase silica Supelcosil LC-SI (Supelco) column (250 mm × 3.0 mm × 3 μm), using hexane:dioxane (97:3 v/v) mixture as eluent (1.2 mL/min) at ambient temperature. The quantification was performed using the internal standard method (tocol).For analysis, an accurate oil amount was weight (30mg), the internal standard added, dissolved in n-hexane, centrifuged (Heraeus Sepatech, Germany) at 4,000 g and transferred to the injection vials. A LOD and LOQ of 1 and 3 mg/100 g oil, respectively, were achieved for the global method, with a linearity from 2 to 100 μg/ml of injected solution.
JFCA-D-14-00314
Fernandes et al.
8
The detection was performed by the fluorescence detector FP2020 Plus model at 290 nm (excitation) and 330 nm (emission) wavelengths. The tocopherols and tocotrienols separation was performed on a normal phase silica Supelcosil LC-SI (Supelco) column (250 mm × 3.0 mm × 3 μm), using hexane:dioxane (97:3 v/v) mixture as eluent (1.2 mL/min) at ambient temperature. The quantification was performed using the internal standard method (tocol).For analysis, an accurate oil amount was weight (30mg), the internal standard added, dissolved in n-hexane, centrifuged (Heraeus Sepatech, Germany) at 4,000 g and transferred to the injection vials. A LOD and LOQ of 1 and 3 mg/100 g oil, respectively, were achieved for the global method, with a linearity from 2 to 100 μg/ml of injected solution.
JFCA-D-14-00314
Fernandes et al.
8
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