Bethanechol chloride (Selleckchem) was dissolved in 0.9% saline. Acetyl-beta-methacholine-chloride (Sigma) was dissolved in 0.9% saline for flexiVent studies.
Acetyl beta methacholine chloride
Manufactured by Merck Group
Acetyl-beta-methacholine-chloride is a chemical compound used in laboratory research. It is a synthetic analogue of the neurotransmitter acetylcholine. This compound is commonly used in various scientific experiments and assays, but its detailed description and intended use should be provided by the scientific experts or research professionals.
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5 protocols using acetyl beta methacholine chloride
1
Flexivent Reagent Preparation
2
Flexivent Acetyl-beta-methacholine Study
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Acetyl-beta-methacholine-chloride (Sigma) was dissolved in 0.9% saline for flexiVent studies.
3
Acetyl-beta-methacholine-chloride Dilution Protocol
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Acetyl‐beta‐methacholine‐chloride (Sigma‐Aldrich) was dissolved in 0.9% saline for ex vivo application. Bay 11‐7082 (Cat. 196871; Sigma‐Aldrich) was diluted in DMSO and used at a final concentration of 10 µM (Kudo et al., 2012 ). Substance P (Sigma‐Aldrich) was dissolved in DMSO and used at a final concentration of 100 nM. Final concentration of DMSO (vehicle controls) was 0.1% (vol/vol).
4
Preparation of Reagents for Mouse Airway Studies
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Acetyl-beta-methacholine-chloride (Sigma) was dissolved in 0.9% saline for flexiVent studies. Mouse IL-13 (R&D Systems, MN, United States) was dissolved in 0.9% sterile saline containing 0.1% BSA carrier to a concentration of 50 μg/mL, aliquoted and stored at −20°C until use. Fresh aliquots were thawed on the day of treatment for intranasal delivery each day. The CREB inhibitor 666–15 (Li et al., 2016 (link)) (R&D Systems) was initially dissolved in 100% DMSO at a concentration of 1 mM. The stock was diluted 1:10,000 into complete growth media for cell culture experiments.
5
Preparation of Cytokine and Inhibitor Solutions
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Acetyl-beta-methacholine-chloride (Sigma) was dissolved in 0.9% saline for flexiVent studies. Mouse IL-1B (R&D Systems, MN, US) was dissolved in 0.9% sterile saline containing 0.1% BSA carrier to a concentration of 10 mg/mL, aliquoted and stored at −20°C until use. A new aliquot of mouse IL-1B (10 mg/mL) stock was diluted 1:1,000 into 0.9% sterile saline for intranasal delivery each day. Recombinant human IL-1B (R&D Systems) was dissolved in PBS to a concentration of 100 mg/mL, aliquoted and stored at −20°C until use. A new aliquot of human IL-1B (100 mg/mL) stock was diluted 1:10,000 into complete growth media for cell culture experiments each day. The CREB inhibitor 666-15 (Li et al., 2016 (link)) (R&D Systems) was initially dissolved in 100% DMSO at a concentration of 1 mM. The stock was diluted 1:10,000 into complete growth media for cell culture experiments.
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