Animals were randomly assigned to one of the following three experimental groups: (1) control (CON), (2) systemic SFN treatment (SFN-S) and (3) platelet specific SFN (SFN-P) treatment. Control animals in group 1 received an intraperitoneal (ip) injection of 10 mL/kg body weight (BW) dissolvent (mix of corn oil and sterilized phosphate buffered saline (PBS), ratio of 1:9)) 24 h prior induction of intestinal IRI. Animals undergoing systemic SFN treatment in group 2 received an ip injection of SFN (5 mg/kg BW, L-Sulphoraphane, Sigma-Aldrich, Darmstadt, Germany, dissolved in a mix of 10% corn oil and 90% PBS) 24 h prior induction of intestinal IRI. For platelet specific SFN treatment in group 3, platelet donor mice received 5 mg/kg BW SFN (Sigma-Aldrich) ip 24 h prior to platelet donation. SFN-P animals were only used for intravital microscopy and are not included in datasets for any downstream assay (for further details see Supplementary Figure S1 ).
L sulphoraphane
Manufactured by Merck Group
Sourced in Germany
L-Sulphoraphane is a chemical compound used in laboratory research and analysis. It is a member of the isothiocyanate family and is derived from cruciferous vegetables. L-Sulphoraphane is utilized in various scientific applications, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.
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2 protocols using l sulphoraphane
1
Systemic and Platelet-Specific SFN in Intestinal IRI
2
Preparation of Bioactive Compound Solutions
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Tert-butylhydroquinone (tBHQ, CAS: 1948-33-0) , diethyl maleate (DEM, CAS: 141-05-9), L-sulphoraphane (CAS: 142825-10-3), and nitrofurantoin (CAS: 67-20-9) were obtained from Sigma-Aldrich. and Bardoxolone methyl (CDDO-Me, CAS: 218600-53-4) was obtained from Cayman Chemicals/Bio-Connect. All compounds were dissolved in 100% dimethyl sulfoxide (DMSO, CAS: 67-68-5) from Sigma-Aldrich, to obtain aliquots with stock concentrations of 0.1 M.
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