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Embutramide

Manufactured by MSD
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Sourced in United States, France

Embutramide is a chemical compound used in laboratory equipment. It serves as a key component in various analytical and research applications. The core function of Embutramide is to facilitate specific processes and reactions within controlled laboratory environments.

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Lab products found in correlation

Embutramide, by MSD animal health (4 mentions) 2 ml cryo tubes, by Sarstedt (1 mentions) Ketamine 1000, by Virbac (1 mentions) Rompun 2, by Bayer (1 mentions) Dolethal, by Vetoquinol (1 mentions) Centrifuge 5810r, by Eppendorf (1 mentions)

5 protocols using embutramide

1

Postoperative Care and Radiographic Monitoring in Pigs

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After surgery, pigs were checked until full recovery from anesthesia. Two units of Amoxicillin/clavulanic acid 15 mg/kg (Amoksiklav, Lek Pharmaceuticals, Ljubljana, Slovenia) were administered every 72 h for one week. Pigs received Ketoprofen analgesics (Comforion Vet, Orion Pharma Animal Health, Espoo, Finland) intramuscularly regularly for one week after surgical procedure.
Pigs were housed individually with free access to water. General and neurological examinations were performed daily during the first week and twice per week later. Plain radiographs of antero-posterior and lateral views were regularly repeated after 2, 4, and 8 weeks after surgery in group A. In group B X–rays were taken after 2, 4, 8, 12, and 16 weeks. Pigs in both groups were euthanized by i.v. administration of Embutramide, Mebezonium iodide, Tetracaine hydrochloride injectable solution at 6ml/50kg (T61, MSD Animal Health, Kenilworth, NJ, USA).
Krticka M., Planka L., Vojtova L., Nekuda V., Stastny P., Sedlacek R., Brinek A., Kavkova M., Gopfert E., Hedvicakova V., Rampichova M., Kren L., Liskova K., Ira D., Dorazilová J., Suchy T., Zikmund T., Kaiser J., Stary D., Faldyna M, & Trunec M. (2021). Lumbar Interbody Fusion Conducted on a Porcine Model with a Bioresorbable Ceramic/Biopolymer Hybrid Implant Enriched with Hyperstable Fibroblast Growth Factor 2. Biomedicines, 9(7), 733.
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2

Porcine Intestinal Digesta Sampling

Cited 1 time
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Intestinal digesta samples were collected 2 h after morning feeding. After sedation (Narketan, 10 ml/kg body weight; Ketamine HCl; Vétoquinol AG, Ittigen, Austria; and Stresnil, 3 ml/kg body weight; Azaperone; Biokema SA, Crissier, Switzerland), pigs were euthanized by intracardiac injection of T61 (10 ml/kg, Embutramide; MSD Animal Health, Vienna, Austria) [9 (link)]. The abdominal cavity was opened. The whole gastrointestinal tract was removed from the abdomen, and the small and large intestines were identified, isolated, and carefully dissected them from the mesentery. The cecum and mid colon (top of the beehive) were identified, opened at the mesentery and emptied. The luminal digesta was collected, thoroughly homogenized and stored on ice until long-term storage at − 80 °C.
Metzler-Zebeli B.U., Newman M.A., Grüll D, & Zebeli Q. (2019). Functional adaptations in the cecal and colonic metagenomes associated with the consumption of transglycosylated starch in a pig model. BMC Microbiology, 19, 87.
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3

Porcine Euthanasia and Fecal Sampling

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On days 18 and 19, pigs were euthanized 2 h after their last feeding via intracardiac injection of T61 (10 ml/kg Embutramide, MSD animal Health, Vienna, Austria) after general anesthesia was induced (Narketan 100 mg/ml, 1 ml/10 kg body weight, Ketaminhydrochlorid, Vétoquinol GmbH, Germany; Stresnil 40 mg/ml; 0.5 ml/10 kg body weight, Azaperon, Elanco Deutschland GmbH, Germany). For the analysis of metabolic active bacteria, fresh feces from the rectum were aseptically collected in 2-ml cryo-tubes (Sarstedt AG & Co., Nümbrecht, Germany), immediately snap-frozen in liquid nitrogen and stored at −80°C until analysis. In one pig, the rectum was empty and digesta could not be collected for microbial analysis.
Klinsoda J., Vötterl J., Zebeli Q, & Metzler-Zebeli B.U. (2019). Lactic Acid Treatment of Cereals and Dietary Phytase Modified Fecal Microbiome Composition Without Affecting Expression of Virulence Factor Genes in Growing Pigs. Frontiers in Microbiology, 10, 2345.
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4

Rabbit Eye Biocompatibility and Biointegration

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After the last examinations were conducted, rabbits were euthanized. In the biocompatibility study, euthanasia of the animals was performed by intravenous injection of 1 mL/kg of pentobarbital (182.20 mg/mL, Dolethal, Vetoquinol, France). Prior to euthanasia, animals were sedated by an intramuscular injection of 10 mg/kg ketamin hydrochloride (Ketamine 1000, Virbac, France) and 2 mg/kg xylazine chlorhydrate (Rompun 2%, Bayer, Germany). In the biointegration study, euthanasia of the animals was performed by intravenous injection of 0.1 mL/kg of T61 (4.39 mg/mL tetracaine chlorhydrate, 26.92 mg/mL mebezonium diiodure, 200.00 mg/mL embutramide, MSD, France). Prior to euthanasia, animals were sedated by an intramuscular injection of 2 mg/kg of xylazine chlorhydrate (Xyl M, V.M.D., Belgium), followed 5 min later by an intramuscular injection of 25 mg/kg ketamin chlorhydrate (Nimatek, Dechra, Netherlands) and 3.5 mg/kg xylazine chlorhydrate (Xyl M, V.M.D., Belgium). Sedation prior to euthanasia was performed for ethical purposes. The eyes were then removed in toto and immersed in appropriate fixative solution for further histopathological processing.
Grierson I., Minckler D., Rippy M.K., Marshall A.J., Collignon N., Bianco J., Detry B, & Johnstone M.A. (2020). A novel suprachoroidal microinvasive glaucoma implant: in vivo biocompatibility and biointegration. BMC Biomedical Engineering, 2, 10.
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5

Porcine Intestinal Tissue Sampling

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On day 15 of the experiment, 3-4 h after receiving fresh feed, animals were anaesthesised (Narketan, 10 ml/kg body weight; Ketamine HCl; Ve ´toquinol AG and Stresnil, 3 ml/kg body weight; Azaperone; Biokema SA); blood was collected by cardiac puncture into serum collection tubes (Primavette; KABE Labortechnik) and placed on ice until further processing. Blood samples were centrifuged at 1811 g for 10 min at 48C (Eppendorf Centrifuge 5810 R; Eppendorf) and stored at 2 208C until analysis. Immediately after blood withdrawal, pigs were euthanised by an intracardiac injection of T61 (10 ml/kg, Embutramide; MSD Animal Health) and exsanguinated. The abdominal cavity was opened and the entire gastrointestinal tract was removed. For candidate gene expression, the mid-jejunum (30 cm in the middle of the jejunum) and mid-colon (the middle of the colon after division into three equal segments) were selected as important intestinal sites for digestion and fermentation, respectively. Gut pieces were opened at the mesentery, and digesta were removed. Gut tissues were thoroughly washed in ice-cold PBS, and the mucosa was scraped off using a glass plate. Mucosa samples were immediately snap-frozen in liquid N 2 and stored at 2808C.
Metzler-Zebeli B.U., Mann E., Ertl R., Schmitz-Esser S., Wagner M., Klein D., Ritzmann M, & Zebeli Q. (2015). Dietary calcium concentration and cereals differentially affect mineral balance and tight junction proteins expression in jejunum of weaned pigs. The British journal of nutrition, 113(7).
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