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Mouse anti cas9

Manufactured by Diagenode
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The Mouse anti-Cas9 is a monoclonal antibody that binds to the Cas9 protein, which is a key component of the CRISPR-Cas9 gene editing system. This antibody can be used for the detection and quantification of Cas9 in various experimental applications.

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Lab products found in correlation

Rabbit anti rnaseh2c, by Proteintech (2 mentions) Rabbit anti rnaseh2a, by OriGene (2 mentions) Mouse anti rnaseh2a, by Abcam (2 mentions) Mouse anti γh2ax jbw301, by Merck Group (2 mentions) Rabbit anti rad51 h 92, by Santa Cruz Biotechnology (2 mentions) Rabbit anti parp1 h 250, by Santa Cruz Biotechnology (2 mentions) Mouse anti par 10h, by Enzo Life Sciences (2 mentions) Rabbit anti topoisomerase 1, by Abcam (2 mentions) Mouse anti α tubulin dm1a, by Merck Group (2 mentions) Rabbit anti dna polymerase beta, by Abcam (2 mentions) Mouse anti rnaseh2a g 10, by Santa Cruz Biotechnology (2 mentions) Rabbit anti dykddddk, by Cell Signaling Technology (2 mentions) Mouse anti α tubulin b512, by Merck Group (2 mentions) Mouse anti vinculin, by Merck Group (2 mentions) Rabbit anti actin, by Merck Group (2 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Rabbit anti gapdh, by Merck Group (2 mentions) Ab16667, by Abcam (2 mentions) Imagescope, by Leica camera (2 mentions) A11122, by Agilent Technologies (2 mentions)

5 protocols using mouse anti cas9

1

Immunoblot Analysis of Cytoskeletal Proteins

Cited 1 time
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Cell lysates and immunoblot analysis were performed as described in (19 (link)). The following antibodies were used: rabbit anti-MRCKα pS1003 antibody raised by Eurogentec using the peptide AcNH-KGCPG-S(PO3H2)-TGFPP-CONH2, rabbit anti-pMLC2 Thr18/Ser19 (#3674; Cell Signaling Technology), mouse anti-MRCL3/MRLC2/MYL9 (sc-28329; Santa Cruz Biotechnology), mouse anti α-tubulin (T9026; Sigma), rabbit anti-ERK2 (Chris Marshall, Institute of Cancer Research, London UK), mouse anti-MRCKα (H00008476-M01; Abnova), mouse anti-MRCKβ (H00009578-M03; Abnova), mouse anti-MRCKαβ (MANDM1 6G8; Glenn Morris, Centre for Inherited Neuromuscular Disease, Oswestry UK) (23 (link)), mouse anti-ROCK1 (BD-611136; BD Biosciences), mouse anti-ROCK2 (BD-61062; BD Biosciences), mouse anti-Cas9 (C15200229; Diagenode), mouse anti-FLAG (F4042; Sigma). Secondary antibodies used were: goat anti-mouse IgG Dylight 800 (35521; Thermo Scientific), goat anti-rabbit IgG AlexaFluor 680 (A12076; Invitrogen), goat anti-rabbit IgG IR Dye 800 CW (926-32211; LI-COR Biosciences), goat anti-mouse IgG IR Dye 680 (926-68020; LI-COR Biosciences).
Unbekandt M., Belshaw S., Bower J., Clarke M., Cordes J., Crighton D., Croft D.R., Drysdale M.J., Garnett M.J., Gill K., Gray C., Greenhalgh D.A., Hall J.A., Konczal J., Lilla S., McArthur D., McConnell P., McDonald L., McGarry L., McKinnon H., McMenemy C., Mezna M., Morrice N.A., Munro J., Naylor G., Rath N., Schüttelkopf A.W., Sime M, & Olson M.F. (2018). Discovery of potent and selective MRCK inhibitors with therapeutic effect on skin cancer. Cancer research, 78(8), 2096-2114.
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2

Antibody Characterization for Protein Detection

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The following antibodies were used for immunofluorescence (IF) and immunoblotting (IB) at indicated dilutions: sheep anti-pan-RNase H2 (raised against human recombinant RNase H213 (link), IB 1:1,000, IP 5 µl / 1 ml lysate); rabbit anti-RNASEH2C (Proteintech 16518-1-AP; IB 1:1,000); rabbit anti-RNASEH2A (Origene TA306706, IB 1:1,000); mouse anti-RNASEH2A (Abcam ab92876; IF 1:500); mouse anti-RNASEH2A G-10 (Santa Cruz Biotechnologies sc-515475; WB 1:1000); mouse anti-γH2AX JBW301 (Millipore 05-636, IF 1:800 – 1:2,000); rabbit anti-RAD51 H-92 (Santa Cruz Biotechnologies sc-8349, IF 1:150); rabbit anti-BRCA146 (link) (IB 1:1,000); mouse anti-Cas9 (Diagenode C15200203, IB 1:1,000); rabbit anti-PARP1 H-250 (Santa Cruz Biotechnologies sc-7150, IB 1:1,000); mouse anti-PAR 10H (Enzo ALX-804-220-R100, IB 1:1,000); rabbit anti-Topoisomerase I (Abcam ab109374; IB 1:5,000); rabbit anti-DYKDDDDK (Cell Signaling Technologies 2368, IB 1:1,000); rabbit anti-actin (Sigma A2066, IB 1:5,000); mouse anti-α–tubulin DM1A (Millipore CP06, IB 1:5,000); mouse anti-α–tubulin B512 (Sigma T6074, IB 1:5,000); rabbit anti-GAPDH (Sigma G9545, IB 1:20,000); mouse anti-vinculin (Sigma V9264, IB 1:1,000); rabbit anti-DNA polymerase beta (Abcam ab26343, IB 1:1,000); rabbit anti-cleaved caspase-3 (Cell Signaling Technologies 9661S, IF 1:800).
Zimmermann M., Murina O., Reijns M.A., Agathanggelou A., Challis R., Tarnauskaitė Ž., Muir M., Fluteau A., Aregger M., McEwan A., Yuan W., Clarke M., Lambros M.B., Paneesha S., Moss P., Chandrashekhar M., Angers S., Moffat J., Brunton V.G., Hart T., de Bono J., Stankovic T., Jackson A.P, & Durocher D. (2018). CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature, 559(7713), 285-289.
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3

Detailed Brain Dissection and Histological Analysis

Cited 2 times
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Mice were sacrificed by carbon dioxide asphyxiation or deep anesthesia with isoflurane followed by cervical dislocation. Mouse brains were manually dissected under a fluorescent stereoscope (Zeiss, Olympus or Leica). Brightfield and/or GFP fluorescent images were taken for the dissected brain, and overlaid using ImageJ58 (link). Brains were then fixed in 4% formaldehyde or 10% formalin for 48 to 96 hours, embedded in paraffin, sectioned at 6 μm and stained with hematoxylin and eosin (H&E) for pathology. For tumor size quantification, H&E slides were scanned using an Aperio digital slidescanner (Leica). Tumors were manually outlined as region-of-interest (ROI), and subsequently quantified using ImageScope (Leica). Sections were de-waxed, rehydrated and stained using standard immunohistochemistry (IHC) protocols as previously55 (link), 59 (link). The following commonly used antibodies were used for IHC: rabbit anti-Ki67 (abcam ab16667, 1:500), rabbit anti-GFP (ThermoFisher Scientific A11122, 1:300) rabbit anti-GFAP (Dako, 1:500), and mouse anti-Cas9 (Diagenode, 1:300).
Chow R.D., Guzman C.D., Wang G., Schmidt F., Youngblood M.W., Ye L., Errami Y., Dong M.B., Martinez M.A., Zhang S., Renauer P., Bilguvar K., Gunel M., Sharp P.A., Zhang F., Platt R.J, & Chen S. (2017). AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma. Nature neuroscience, 20(10), 1329-1341.
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4

Detailed Brain Dissection and Histological Analysis

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were sacrificed by carbon dioxide asphyxiation or deep anesthesia with isoflurane followed by cervical dislocation. Mouse brains were manually dissected under a fluorescent stereoscope (Zeiss, Olympus or Leica). Brightfield and/or GFP fluorescent images were taken for the dissected brain, and overlaid using ImageJ58 (link). Brains were then fixed in 4% formaldehyde or 10% formalin for 48 to 96 hours, embedded in paraffin, sectioned at 6 μm and stained with hematoxylin and eosin (H&E) for pathology. For tumor size quantification, H&E slides were scanned using an Aperio digital slidescanner (Leica). Tumors were manually outlined as region-of-interest (ROI), and subsequently quantified using ImageScope (Leica). Sections were de-waxed, rehydrated and stained using standard immunohistochemistry (IHC) protocols as previously55 (link), 59 (link). The following commonly used antibodies were used for IHC: rabbit anti-Ki67 (abcam ab16667, 1:500), rabbit anti-GFP (ThermoFisher Scientific A11122, 1:300) rabbit anti-GFAP (Dako, 1:500), and mouse anti-Cas9 (Diagenode, 1:300).
Chow R.D., Guzman C.D., Wang G., Schmidt F., Youngblood M.W., Ye L., Errami Y., Dong M.B., Martinez M.A., Zhang S., Renauer P., Bilguvar K., Gunel M., Sharp P.A., Zhang F., Platt R.J, & Chen S. (2017). AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma. Nature neuroscience, 20(10), 1329-1341.
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5

Antibody Characterization for Protein Detection

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for immunofluorescence (IF) and immunoblotting (IB) at indicated dilutions: sheep anti-pan-RNase H2 (raised against human recombinant RNase H213 (link), IB 1:1,000, IP 5 µl / 1 ml lysate); rabbit anti-RNASEH2C (Proteintech 16518-1-AP; IB 1:1,000); rabbit anti-RNASEH2A (Origene TA306706, IB 1:1,000); mouse anti-RNASEH2A (Abcam ab92876; IF 1:500); mouse anti-RNASEH2A G-10 (Santa Cruz Biotechnologies sc-515475; WB 1:1000); mouse anti-γH2AX JBW301 (Millipore 05-636, IF 1:800 – 1:2,000); rabbit anti-RAD51 H-92 (Santa Cruz Biotechnologies sc-8349, IF 1:150); rabbit anti-BRCA146 (link) (IB 1:1,000); mouse anti-Cas9 (Diagenode C15200203, IB 1:1,000); rabbit anti-PARP1 H-250 (Santa Cruz Biotechnologies sc-7150, IB 1:1,000); mouse anti-PAR 10H (Enzo ALX-804-220-R100, IB 1:1,000); rabbit anti-Topoisomerase I (Abcam ab109374; IB 1:5,000); rabbit anti-DYKDDDDK (Cell Signaling Technologies 2368, IB 1:1,000); rabbit anti-actin (Sigma A2066, IB 1:5,000); mouse anti-α–tubulin DM1A (Millipore CP06, IB 1:5,000); mouse anti-α–tubulin B512 (Sigma T6074, IB 1:5,000); rabbit anti-GAPDH (Sigma G9545, IB 1:20,000); mouse anti-vinculin (Sigma V9264, IB 1:1,000); rabbit anti-DNA polymerase beta (Abcam ab26343, IB 1:1,000); rabbit anti-cleaved caspase-3 (Cell Signaling Technologies 9661S, IF 1:800).
Zimmermann M., Murina O., Reijns M.A., Agathanggelou A., Challis R., Tarnauskaitė Ž., Muir M., Fluteau A., Aregger M., McEwan A., Yuan W., Clarke M., Lambros M.B., Paneesha S., Moss P., Chandrashekhar M., Angers S., Moffat J., Brunton V.G., Hart T., de Bono J., Stankovic T., Jackson A.P, & Durocher D. (2018). CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature, 559(7713), 285-289.
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