Ca(NO3)2•4H2O, (NH4)2HPO4, anhydrous ethanol, and ammonia solution were purchased from Sinopharm Chemical Reagent Co. (Beijing, China). HepG2 and human hepatocyte (L-02) cells were provided by China Center for Type Culture Collection. DMEM medium, RPMI-1640, phosphate buffered saline (PBS), penicillin-streptomycin, and trypsin-EDTA were purchased from HyClone. Fetal bovine serum (FBS) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were provided by Tianhang Biotechnology Co. (Zhejiang, China). 2‘,7‘-dichlorofluorescein diacetate (DCFH/DA), propidium iodide (PI), Hoechst33342, HRP-conjugated IgG, Caspase-3, 8, and 9 fluorescence assay kits were purchased from Beyotime Institute of Biotechnology (Haimen, China).
Nh4 2hpo4
Manufactured by Sinopharm
Sourced in China
(NH4)2HPO4 is a chemical compound that is commonly known as ammonium phosphate dibasic. It is a white crystalline solid that is soluble in water. The compound's primary function is as a chemical reagent, and it is used in various applications, such as in the production of fertilizers, fire retardants, and as a food additive.
Automatically generated - may contain errors
Lab products found in correlation
Ca no3 2 4h2o, by Sinopharm (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Anhydrous ethanol, by Sinopharm (1 mentions)
Ammonia solution, by Sinopharm (1 mentions)
Selenocystine, by Merck Group (1 mentions)
Selenomethionine, by Merck Group (1 mentions)
Protease k, by Merck Group (1 mentions)
Hcooh, by Sinopharm (1 mentions)
Se methylselenocysteine, by Merck Group (1 mentions)
Protease xiv, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Sinopharm (1 mentions)
Hydrochloric acid (hcl), by Sinopharm (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Sinopharm (1 mentions)
Ammonium hydroxide, by Sinopharm (1 mentions)
Fluorescein diacetate, by Merck Group (1 mentions)
Iscript gdna clear cdna synthesis kit, by Bio-Rad (1 mentions)
Trypsin edta 0.25, by Thermo Fisher Scientific (1 mentions)
Annexin 5 fluorescein isothiocyanate fitc apoptosis detection kit 1, by BD (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
7 protocols using nh4 2hpo4
1
Cytotoxicity of Calcium Nitrate and Ammonium Phosphate
2
Selenocompound Analysis in Biological Samples
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Deionized water (Millipore system) was used in this study for chemical preparation. All chemicals used (e.g., Tris, HCl, H2O2, HCOOH, NaOH and (NH4)2HPO4) were analytical grade or above, provided by Sinopharm Chemical Reagent (Shanghai, China). A 1000 mg/L Se stock standard solution was purchased from Merck Millipore (Darmstadt, Germany). Besides, Protease K (30 U/mg), Protease XIV (5.5 U/mg), selenomethionine (SeMet, >98%), selenocystine (SeCys2, >98%), and Se-methylselenocysteine (SeMeSeCys, >98%) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
3
Calcium Phosphate Nanoparticle Synthesis
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Ca(NO3)2·4H2O (AR, Sinopharm Chemical Reagent, Shanghai, China), (NH4)2HPO4 (AR, Sinopharm Chemical Reagent, Shanghai, China), CTAB (AR, Sinopharm Chemical Reagent, Shanghai, China), and EDTA (AR, Sinopharm Chemical Reagent, Shanghai, China) were used as received without further purification.
4
Keratin-Based Biomaterial Synthesis
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The partially hydrolyzed keratin was purchased from Yuanchen Co., Ltd. (Wuhan, China). Ca(NO3)2·4H2O, (NH4)2HPO4 and ammonium hydroxide were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). All of the reagents were used as received without treatment or further purification.
5
Melanoma Cell Cytotoxicity Assay
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Ca(NO3)2·4H2O, (NH4)2HPO4, and NH3·H2O were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). PEG2000 was purchased from the Aladdin (Shanghai, China). Human melanoma cells (A375) and human epidermal fibroblasts (HSF) were purchased from iCell Bioscience Inc (Shanghai, China). FBS, DMEM medium, penicillin–streptomycin solution, PBS, and Trypsin 0.25% EDTA were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fluorescein diacetate and propidium iodide (PI) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). DAPI was purchased from Beyotime (Shanghai, China). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumanoto, Japan). Fluorescein isothiocyanate (FITC) Annexin V Apoptosis Detection Kit I was purchased from BD (Franklin Lakes, NJ, USA). The RNeasy Mini Kit was purchased from Qiagen (Hilden, Germany). The iScript™ gDNA Clear cDNA Synthesis kit and SsoFast™ EvaGreen Supermix were purchased from Bio-Rad Laboratories Inc. (Hercules, CA, USA). The Matrigel was purchased from Corning Incorporated (Corning, NY, USA).
6
Phosphatidylserine-Targeted Nanoparticle Delivery
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Phosphatidylserine (PS) was purchased from Shanghai Yiji Medicine & Chemical Co, Ltd. Boc-NH-PEG-COO-NHS, Poly-(ethylene glycol) methyl ether (PEG, Mn = 2000), Rb (rhodamine b) and CaCl2 were obtained from Sigma-Aldrich, and RGD was purchased from Corner Stone Therapeutics (Shanghai), Ltd (Shanghai, China).
VRP was purchased from Melonepharma Co, Ltd, Dalian. NVT was purchased from Chongqing Carelife Pharmaceutical Co, Ltd. (NH4)2HPO4 was obtained from Sinopharm Chemical Reagent Co., Ltd.
MCF7/MDR cells (MDR human breast cancer cells) and MCF7 cells were obtained from the Institute of Biochemistry and Cell Biology at the Chinese Academy of Sciences (Shanghai, China). BALB/c nude mice were provided from the Animal Experiment Centre of Shanghai Cancer Institute.
VRP was purchased from Melonepharma Co, Ltd, Dalian. NVT was purchased from Chongqing Carelife Pharmaceutical Co, Ltd. (NH4)2HPO4 was obtained from Sinopharm Chemical Reagent Co., Ltd.
MCF7/MDR cells (MDR human breast cancer cells) and MCF7 cells were obtained from the Institute of Biochemistry and Cell Biology at the Chinese Academy of Sciences (Shanghai, China). BALB/c nude mice were provided from the Animal Experiment Centre of Shanghai Cancer Institute.
7
Quantitative Proteomics Analysis of Cellular Responses
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Glucose, tryptone, agar, and TEAB were purchased from Sangon Biotech Co. Ltd. (Shanghai, China). Glycerin, (NH 4 ) 2 HPO 4 , urea, acetone, and isopropanol were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). DTT, PMSF, EDTA, and acetonitrile (HPLC grade) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Trypsin was obtained from Takara Co., Ltd. (Kyoto, Japan). iTRAQ Reagent 8 Plex One Assay Kit was purchased from SCIEX (Scientific Export).
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