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2 protocols using naringin

1

Polyphenol Compound Procurement for Biochemical Assays

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Flavone, 6-hydroxy flavone, 3-hydroxy flavone, 7-hydroxy flavone, 6-methoxy-flavone, 6-methoxy flavanone, naringenin and naringin were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Biochanin A, genistein, apigenin, puerarin, wogonin, catechin (C), epicatechin (EC), luteolin, daidzein, daidzin, resveratrol, piceid and neohesperidin-dihydrochalcone were purchased from Aladin Co. Ltd. (Shanghai, China). Galangin, kaempferol, quercetin, myricetin, baicalein and chrysin were purchased from Wako Pure Chemical Industries (Osaka, Japan). All other polyphenols were obtained commercially from Shanghai Tauto Biotech CO., Ltd. (Shanghai, China). Peptone, yeast extract, agar, 1-deoxy-1-morpholino fructose (DMF) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nitrotetrazolium Blue chloride (NBT) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Kanamycin and carbenicillin were purchased from Shanghai Xinya Pharmaceutical Co., Ltd. (Shanghai, China). Fetal bovine serum (BP) of no phage and low endotoxin was from Sijiqing Biological Engineering Materials Co., Ltd. (Hangzhou, China). All other reagents and solvents were analytical grade and used without further purification.
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2

Anti-PD-L1 Antibody and Compound Assay

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Anti‐programmed death‐ligand 1 (PD‐L1) antibody and IgG controls were purchased from BioLegend. Diphtheria toxin was obtained from FUJIFILM Wako Pure Chemical Corporation. Naringin and naringenin (Tokyo Chemical Industry) were dissolved in dimethyl sulfoxide at a concentration of 100 mM.
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