Recombinant (CXCL12) SDF-1α was purchased from Pepro Tech (USA). The CXCR4 antagonist AMD3100, PHA-665752 and Nystatin were obtained from Sigma (St. Louis, MO). Stattic was obtained from Selleck. Anti-CXCR4 antibodies were obtained from ABCAM. Anti–E-cadherin, anti-Vimentin, anti-ZEB1, anti-STAT3, anti–phosphor-STAT3, and antibodies to c-MET and phospho-c-MET (Tyr1234/1235) were purchased from Cell Signaling Technology (Beverly, MA). Anti-Snail and anti-Twist2 were purchased from Abcam (Cambridge, MA). Anti–Cav-1 and anti–phospho-Cav-1-1 (Tyr14) antibodies were obtained from BD Technology Co. (USA). All the other antibodies were purchased from Santa Cruz Biotechnology (USA).
Anti phosphor stat3
Manufactured by Cell Signaling Technology
Anti–phosphor-STAT3 is a laboratory reagent used to detect and quantify the phosphorylated form of the STAT3 transcription factor. STAT3 is a key regulator of cellular processes, and its phosphorylation status is an important indicator of its activation.
Automatically generated - may contain errors
Lab products found in correlation
Anti cxcr4 antibody, by Abcam (1 mentions)
Nystatin, by Merck Group (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Amd3100, by Merck Group (1 mentions)
Anti snail, by Abcam (1 mentions)
Pha 665752, by Merck Group (1 mentions)
Anti zeb1, by Cell Signaling Technology (1 mentions)
Anti stat3, by Cell Signaling Technology (1 mentions)
Recombinant cxcl12 sdf 1α, by Thermo Fisher Scientific (1 mentions)
Stattic, by Selleck Chemicals (1 mentions)
Anti vimentin, by Cell Signaling Technology (1 mentions)
Anti twist2, by Abcam (1 mentions)
Amersham imager 680, by GE Healthcare (1 mentions)
Ripa buffer, by Thermo Fisher Scientific (1 mentions)
Polyvinylidene fluoride membrane, by Bio-Rad (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Anti pparγ, by Abcam (1 mentions)
Anti phosphor nfκb p65, by Cell Signaling Technology (1 mentions)
Anti il 1β, by Abcam (1 mentions)
Anti gapdh, by Abcam (1 mentions)
2 protocols using anti phosphor stat3
1
Signaling Pathways in Metastatic Regulation
2
Western Blot Analysis of Lung Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Lung tissue was lysed by using RIPA buffer (Thermo Fisher Scientific). Equal amounts of proteins were separated by 10% sodium dodecyl sulphate‐polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Bio‐Rad Laboratories). The membrane was blocked with 5% bovine serum albumin (Sigma‐Aldrich) for 90 min at room temperature and incubated with a primary antibody for 14 h at 4℃. The primary antibodies included anti–PPAR‐γ (Abcam), anti‐TGF‐β1 (ProteinTech), anti‐phosphor‐Smad3 (Cell Signaling Technology), anti‐Smad3 (Cell Signaling Technology), anti‐phosphor‐Stat6 (Cell Signaling Technology), anti‐Stat6 (Santa Cruz Biotechnology), anti‐phosphor‐Stat3 (Cell Signaling Technology), anti‐Stat3 (Santa Cruz Biotechnology), anti‐β‐tubulin (ProteinTech), anti‐NFκB P65 (Abcam), anti‐IκBα (Abcam), anti‐IL‐1β (Abcam), anti‐phosphor‐NFκB P65 (Cell Signaling Technology), anti‐phosphor‐IκBα (Cell Signaling Technology) and anti‐GAPDH (Abcam). And then, membranes were incubated with anti‐rabbit/mouse IgG (H+L) (Abcam) for 1 h at room temperature. Chemiluminescence measurement was performed by using Amersham Imager 680 (GE Healthcare Life Science).
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