Anti β arrestin2

The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and anti-β-arrestin2 antibodies were from Cell Signaling Technology (Boston, MA, USA); anti-E-cadherin, anti-N-cadherin, anti-snai1, and anti-β-actin antibodies were purchased from Santa Cruz (Dallas, TX, USA).
N-ethylmaleimide (NEM, deubiquitinase inhibitor), SDF-1 (SDF-1α, CXCR4 agonist), AMD3100 octahydrochloride hydrate (1,1′-[1,4-Phenylenebis(methylene)]bis-1,4,8,11-tetraazacyclotetradecane octahydrochloride, CXCR4 antagonist), dynasore hydrate (3-Hydroxy-naphthalene-2-carboxylic acid (3,4-dihydroxy-benzylidene)-hydrazide hydrate, dynamin inhibitor), HA affinity beads, and Flag affinity beads were all purchased from Sigma-Aldrich.

RoMS (Batch number: 20180902) were provided by Shandong Luye Pharmaceutical Co., Ltd. (Shandong, China). LPS (from Escherichia coli 011: B4, Catalog No: L2640) was purchased from Sigma Aldrich (St. Louis, MO, United States). Dexamethasone (DXM) (CAS No.: 50-02-2) was purchased from Sigma Aldrich (St. Louis, MO, United States). R121 (CAS No.:98,185-20-7), a specific DRD2 antagonist, was purchased from Sigma Aldrich (St. Louis, MO, United States). The ELISA kits of TNF-α and IL-6 were provided by Suolaibao, Inc. (Beijing, China). The biochemical kits of AST and ALT were purchased from Nanjing Jiancheng Co., Ltd. (Jiangsu, China). The primary antibodies used in this study are as follows: anti-TLR4 and anti-IL-6 (Santa Cruz Biotechnology, Dallas, TX, United States), anti-dopamine D2 receptor (Abcam, Cambridge, MA, United States), Anti-β-arrestin2, anti-p-Akt, anti-Akt, anti-p-IκBα, anti-IκBα, anti-p-NF-κBp65, anti-NF-κBp65 (Cell Signaling Technology, Danvers, MA, United States), anti-GAPDH (Proteintech Group, Inc., Wuhan, China). BCA protein assay kit and chemiluminescence plus reagents were purchased from Beyotime Biotechnology (Shanghai, China).