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Dneasy plant mini prep dna extraction kit

Manufactured by Qiagen
Sourced in United States

The DNeasy® Plant Mini-prep DNA Extraction Kit is a laboratory tool designed to extract and purify DNA from plant samples. It provides a standardized and efficient method for obtaining high-quality DNA from a variety of plant tissues.

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2 protocols using dneasy plant mini prep dna extraction kit

1

Tall Fescue Genomic DNA Extraction

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Young leaf tissue from the 205 tall fescue genotypes was collected in a brown paper envelop, freeze-dried, and ground to a fine powder using a Mixer Mill Type MM 300 (Retsch, Hann, Germany). Genomic DNA was extracted using a DNeasy® Plant Mini-prep DNA Extraction Kit (QIAGEN Inc., Valencia, CA, United States) according to the manufacturer instructions. DNA concentrations were quantified using a NanoDrop ND 1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, United States).
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2

Leaf Tissue DNA Extraction Protocol

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From each progeny, young leaf tissue from a clonal copy kept in the greenhouse was collected and placed in a 2-mL tube and immediately frozen in liquid nitrogen. Each sample was ground to a ine powder using a Mixer Mill Type MM 300 (Retsch GmbH). he DNA was extracted using a DNeasy Plant Mini-prep DNA Extraction Kit (QIAGEN, Inc.) according to manufacturer's instructions with the following modiications. he extraction solution used was 500 L AP1 lysis buffer, 5 L RNase A, 165 L AP2 bufer, and 120 L AE. DNA concentrations were quantiied using a NanoDrop ND1000 spectrophotometer (NanoDrop Technologies).
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