A human normal hepatocyte cell line (HHL-5) and HCC cell lines (MHCC97-H, SK-Hep-1, Huh-7 and Hep3b) were purchased from BioVector NTCC, Inc. HHL-5 and HCC cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 U/ml streptomycin at 37°C with 5% CO2 and saturated humidity. After resuscitation, cells of the 4 to 10th generation were used for subsequent experiments.
Mhcc97 h
Manufactured by BioVector
Sourced in China
MHCC97-H is a human liver cancer cell line used for in vitro research. It is a well-established model system for studying liver cancer biology and evaluating potential therapeutic agents.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mhcc97 h
1
Hepatocyte and HCC Cell Cultivation
2
Cell Line Culture of Liver Cells
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Liver epithelial cell-line THLE-2 and HCC cell lines (HuH7, Hep3B, MHCC97-H, LM3) were commercially bought from BioVector NTCC Inc. (Beijing, China). Cell lines were incubated with Roswell Park Memorial Institute-1640 (RPMI-1640; KeyGen, Nanjing, China) containing 10% new-born bovine serum and 1% penicillin-streptomycin. Cells growth was performed in a 5% CO2 incubator at 37°C.
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