Log In Sign up
The largest database of trusted experimental protocols

Standard sandwich elisa protocols

Manufactured by Thermo Fisher Scientific
Visit Supplier

Standard sandwich ELISA protocols provide a reliable method for the quantitative detection and measurement of target analytes in biological samples. This lab equipment utilizes an antibody-based approach to capture and detect the analyte of interest, enabling accurate and precise quantification.

Automatically generated - may contain errors

Lab products found in correlation

Versamax microplate reader, by Molecular Devices (1 mentions)

Close spelling variants of this product

Standard sandwich ELISA Sandwich ELISA ELISAs

2 protocols using standard sandwich elisa protocols

1

Quantifying Cytokine and IgE Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Levels of IL-31, IL-6, OSM and total IgE in peripheral blood or BALF were measured using ELISA kits (R&D Systems). Levels of IFN-γ, IL-4, IL-13 and IL-17 in supernatants were detected using standard sandwich ELISA protocols (eBioscience). Quantification was performed by regression analysis from a standard curve generated from molecule standards included in the kits.
Huang J., Yue H., Jiang T., Gao J., Shi Y., Shi B., Wu X, & Gou X. (2019). IL-31 plays dual roles in lung inflammation in an OVA-induced murine asthma model. Biology Open, 8(1), bio036244.
+ Open protocol
+ Expand
2

Quantifying Lung Inflammation Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols
BAL supernatants were analyzed for Ym1 using commercially available ELISA kits (R&D Systems). Lung homogenates were assayed for IL-4 using standard sandwich ELISA protocols (eBioscience). Analytes were detected using HRP-conjugated streptavidin and TMB substrate (BioLegend) and stopped with 1 M HCl. Final absorbance at 450 nm was measured using a VersaMax microplate reader (Molecular Devices).
Chenery A.L., Alhallaf R., Agha Z., Ajendra J., Parkinson J.E., Cooper M.M., Chan B.H., Eichenberger R.M., Dent L.A., Robertson A.A., Kupz A., Brough D., Loukas A., Sutherland T.E., Allen J.E, & Giacomin P.R. (2019). Inflammasome-Independent Role for NLRP3 in Controlling Innate Antihelminth Immunity and Tissue Repair in the Lung. The Journal of Immunology Author Choice, 203(10), 2724-2734.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!