Spermatocyte or mouse embryonic fibroblast spreads were treated with RNase A (100 mg/mL) at 37°C for 30 min in 2× saline sodium citrate (SSC), denatured at 85°C for 10 min with Cy3-labeled TelC (CCCTAA)3 PNA probe (Panagene), and hybridized for 4 h at 37°C. Preparations were washed twice in 50% formamide/0.5× SSC and then washed twice in 1× SSC at 42°C for 5 min each time.
Cy3 labeled telc ccctaa 3 pna probe
Manufactured by Panagene
Cy3-labeled TelC (CCCTAA)3 PNA probe is a synthetic oligonucleotide probe labeled with the fluorescent dye Cy3. The probe is designed to target the telomeric DNA sequence (CCCTAA)3, which is found at the ends of eukaryotic chromosomes. The Cy3 label allows for the detection of the probe using fluorescence-based techniques.
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Lab products found in correlation
2 protocols using cy3 labeled telc ccctaa 3 pna probe
1
Telomere Detection in Spermatocyte and Fibroblast
2
Telomere Detection in Spermatocyte and Fibroblast
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