Pooled cell-free urine samples of 100 mL were centrifuged at 20,000 ×g (Rotor JA25.50, Beckman Coulter’s Avanti J High-speed Centrifuge) for 20 minutes at 25 °C to get MV-enriched pellet, where apoptotic bodies and exosomes could be coprecipitated. The resulting pellet was fixed overnight in 2.5% glutaraldehyde at room temperature, washed three times with PBS, postfixed in 2% OsO4 at 4 °C for 2 h and then washed twice with double distilled water. After dehydrated in graded acetone, the pellets were embedded in resin-812. Sections of 70 nm were prepared on copper grids and visualized on a JEM-100CX TEM microscope (JEOL).
Jem 100cx tem microscope
Manufactured by JEOL
The JEM-100CX TEM microscope is a transmission electron microscope manufactured by JEOL. It is designed to provide high-resolution imaging of microscopic samples. The JEM-100CX TEM is capable of magnifying samples up to 1,000,000 times and can be used to analyze the internal structure and composition of a wide range of materials.
Automatically generated - may contain errors
2 protocols using jem 100cx tem microscope
1
Isolation and Characterization of Urinary Extracellular Vesicles
2
Collagen and CMA Self-Assembly Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Collagen and CMA suspensions
were made as described above in microfuge tubes and placed in the
incubator at 37 °C to self-assemble for 30 min. One sample of
CMA was placed at 4 °C to disassemble for 30 min and then placed
back in the incubator to allow for reassembly for 30 min. A 10 μL
sample of the supernatant of the gel was placed on a Petri dish, and
an extra-thick carbon 300 mesh copper grid (Electron Microscopy Sciences)
was placed face-down on top of each droplet for 5 min. Filter paper
was used to remove the sample from the TEM grid. To stain, a 10 μL
droplet of 1% phosphotungstic acid (Electron Microscopy Sciences)
was placed on a Petri dish, and the TEM grid was placed face-down
on top of the droplet for 5 min. Filter paper was used to remove the
staining agent, and the samples were dried overnight prior to imaging.
TEM studies were carried out using a JEM-100CX TEM microscope (JEOL).
were made as described above in microfuge tubes and placed in the
incubator at 37 °C to self-assemble for 30 min. One sample of
CMA was placed at 4 °C to disassemble for 30 min and then placed
back in the incubator to allow for reassembly for 30 min. A 10 μL
sample of the supernatant of the gel was placed on a Petri dish, and
an extra-thick carbon 300 mesh copper grid (Electron Microscopy Sciences)
was placed face-down on top of each droplet for 5 min. Filter paper
was used to remove the sample from the TEM grid. To stain, a 10 μL
droplet of 1% phosphotungstic acid (Electron Microscopy Sciences)
was placed on a Petri dish, and the TEM grid was placed face-down
on top of the droplet for 5 min. Filter paper was used to remove the
staining agent, and the samples were dried overnight prior to imaging.
TEM studies were carried out using a JEM-100CX TEM microscope (JEOL).
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