Aypgkf

All reagents were purchased from Thermo Fisher Scientific unless otherwise stated. Chrono-lume, used for the detection of secreted ATP, was purchased from Chrono-log corporation. Anti-pSyk Y525/526 (mouse Y519/520) and anti-pPLCγ2 Y1217 were purchased from Cell Signaling Technology. Anti-pSyk Y352 (Y346 in mice) and anti-pSyk Y348 (Y342 in mice) were purchased from Abcam. Anti-Syk and anti-PLCγ2 were purchased from Santa Cruz Biotechnology. Ibrutinib was purchased from Selleckchem. Odyssey blocking buffer and secondary antibodies IRDye 800CW goat anti-rabbit and IRDye 680LT goat anti-mouse were purchased from Li-Cor. CRP-XL was purchased from Dr Richard Farndale at the University of Cambridge. AYPGKF was purchased from GenScript.

All reagents were purchased from Thermo Fisher Scientific unless otherwise stated. 2-MeSADP, ADP, and Apyrase (type V) were purchased from Sigma (St. Louis, MO, USA). AR-C69931MX was a gift from the Medicines Company (Parsippany, NJ, USA). AYPGKF was purchased from GenScript (Piscataway, NJ, USA). Phosphorylated Akt S473 and phosphorylated VASP S157 were purchased from Cell Signaling Technologies (Beverly, MA, USA), while the total Akt antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The total VASP antibody was purchased from Origene (Rockville, MD, USA). Odyssey blocking buffer and secondary antibodies IRDye 800CW goat anti-rabbit and IRDye 680LT goat anti-mouse were purchased from Li-Cor (Lincoln, NE, USA). Collagen and Chronolume, used for the detection of secreted ATP, were purchased from Chrono-Log Corporation (Havertown, PA, USA). GTP-bound Rap1b was assessed using a kit from Cell Signaling and cAMP production was measured using a kit from Enzo. Prostaglandin E1 was also purchased from Enzo (New York, NY, USA). AseI was purchased from New England BioLabs (Ipswitch, MA, USA).

All reagents were purchased from Thermo Fischer Scientific unless otherwise stated. Collagen and Chronolume, used for the detection of secreted ATP, were purchased from Chrono-log Corporation. The CLEC-2 activating antibody was purchased from Biolegend. Odyssey blocking buffer and secondary antibodies IRDye 800CW goat anti-rabbit and IRDye 680LT goat anti-mouse were purchased from Li-Cor. CRP-XL was purchased from Dr Richard Farndale at the University of Cambridge. AYPGKF was purchased from GenScript. The restriction enzyme SspI was purchased from New England BioLabs.