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3 protocols using anti rps6 phospho ser235 236

1

Signaling Pathway Regulation Protocol

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Anti-TSC2, anti-STAT3 phospho-Ser727 and Tyr705, Ref-1, Rel-A phospho-Ser365, anti-β-actin, VEGF-A, BNIP3, and anti-rpS6 phospho-Ser235/236 were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-HIF-1α was purchased from BD Transduction Laboratories (Oxford, UK). Rapamycin, FL3331, and JSH23 were bought from Merck (Darmstadt, Germany), and KU-0063794 was purchased from Chemquest Ltd. (Cheshire, UK), while APX3330, APX2009, APX2011, and RN7-58 were obtained from Apexian Pharmaceuticals (Indianapolis, IN, USA). Unless stated otherwise, all other lab chemicals were obtained from Merck.
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2

Intracellular Signaling Pathway Analysis

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Anti-myc, anti-rpS6 phospho-Ser235/236, anti-eIF4E, anti-4E-BP1 phospho-Ser65 and Thr36/45, anti-STAT3 Ser727 and Tyr705, and anti-β-actin antibodies were obtained from Cell Signalling Technology. Anti-Rheb was obtained from Santa Cruz (Heidelberg, Germany), anti-HA was obtained from Roche (Hertfordshire, UK), and Anti-HIF-1α was obtained from BD transduction laboratories (Oxford, UK). Rapamycin was obtained from Calbiochem (Nottingham, UK). Dimethyloxallyl Glycine (DMOG) was purchased from R & D systems (Minneapolis, USA). KU-0063794 was purchased from Chemquest Ltd. (Cheshire, UK). Cycloheximide, insulin, PF-4708671, 5,15-DPP, FLLL31 and all other general lab chemicals were obtained from Sigma-Aldrich unless otherwise stated.
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3

Intracellular Signaling Pathway Analysis

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Anti-myc, anti-rpS6 phospho-Ser235/236, anti-eIF4E, anti-4E-BP1 phospho-Ser65 and Thr36/45, anti-STAT3 Ser727 and Tyr705, and anti-β-actin antibodies were obtained from Cell Signalling Technology. Anti-Rheb was obtained from Santa Cruz (Heidelberg, Germany), anti-HA was obtained from Roche (Hertfordshire, UK), and Anti-HIF-1α was obtained from BD transduction laboratories (Oxford, UK). Rapamycin was obtained from Calbiochem (Nottingham, UK). Dimethyloxallyl Glycine (DMOG) was purchased from R & D systems (Minneapolis, USA). KU-0063794 was purchased from Chemquest Ltd. (Cheshire, UK). Cycloheximide, insulin, PF-4708671, 5,15-DPP, FLLL31 and all other general lab chemicals were obtained from Sigma-Aldrich unless otherwise stated.
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