Anti klrg 1 clone 2f1

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anti-KLRG-1 (clone 2F1) is a laboratory reagent used in research applications. It is an antibody that specifically binds to the KLRG-1 protein. KLRG-1 is a cell surface receptor expressed on certain immune cells. The core function of this product is to enable the detection and study of KLRG-1 in various research contexts.

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Anti-KLRG-1 (4 citations) Anti-KLRG-1-APC (clone 2F1; (3 citations)

2 protocols using anti klrg 1 clone 2f1

Analyzing Antigen-specific CD8 T Cells

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Antigen-specific CD8 T cells were identified by labeling with D^b/NP₃₆₆ or D^b/PA₂₂₄in house prepared tetramers for 1 h at 4°C (22 (link)). Tetramer staining was followed by surface staining with appropriate antibody cocktails for 20 min at 4°C. Surface markers were stained using following antibodies: anti-CD8 (clone 53-6.7, BioLegend), anti-CD90.2 (clone 30-H12, BioLegend), anti-CD45.2 (clone 104, BioLegend), anti-CD103 (clone 2E7, BioLegend), anti-CD69 (clone H.12F3, BioLegend), anti-KLRG-1 (clone 2F1, eBioscience, San Diego, CA, USA), anti-CD127 (clone A7R34, BioLegend), anti-CX3CR1 (clone SA011F11, BioLegend), anti-CXCR3 (clone CXCR3-173, BioLegend), and anti-CD49a (clone Ha31/8, BD Pharmingen). Intracellular cytokine staining was performed using anti-IFNγ (clone XMG1.2, BioLegend), anti-TNF (clone MP6-XT22, BioLegend), and anti-IL2 (clone JES6-5H4, BioLegend) antibodies. Proliferation of CD8 T cells was assessed by intracellular staining with anti-Ki67 (clone MOPC-21, BD Pharmingen). Flow cytometry data were acquired using LSRFortessa (Becton Dickinson, Rutherford, NY, USA) and analyzed using the FlowJo software (Tree Star Inc., Ashland, OR, USA).

Van Braeckel-Budimir N., Martin M.D., Hartwig S.M., Legge K.L., Badovinac V.P, & Harty J.T. (2017). Antigen Exposure History Defines CD8 T Cell Dynamics and Protection during Localized Pulmonary Infections. Frontiers in Immunology, 8, 40.

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Multiparametric Flow Cytometry Analysis

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Blood or brain single-cell suspensions were washed in PBS supplemented with 1% FBS, 2 mM EDTA and 0.01% sodium azide, and incubated for 10 min at 4 • C with culture supernatant from the hybridoma cell line 2.4G2 to block Fc receptors. The cells were surface stained with fluorochrome-conjugated monoclonal antibody for 15 min at 4 • C. LIVE/DEAD fixable blue dye (Life Technologies) was used to exclude dead cells. The following antibodies were used: anti-CD45.1 (clone A20, BioLegend), anti-CD11b (clone M1/70, BD), anti-CD19 (clone 6D5, BioLegend), anti-CD3 (clone 145-2C11, BioLegend), anti-CD4 (clone RM4-5, BD), anti-CD8 (clone 53-6.7, BD or Biolegend), anti-FOXP3 (clone FJK-16s, eBioscience), anti-CD44 (clone IM7, BioLegend), anti-CD62L (clone MEL-14, BD), anti-CD127 (clone A7R34, BioLegend) and anti-KLRG1 (clone 2F1, eBioscience). OVA-specific CD8 + T cells were detected using H-2K b /SIINFEKL pentamers (ProImmune). Intracellular FoxP3 staining was performed using BioLegend FOXP3 Fix/Perm Buffer Set. Samples were acquired on LSRII Flow Cytometer (BD), and the data were analyzed using FlowJo software (TreeStar Inc.).

Grasso C., Field C.S., Tang C.W., Ferguson P.M., J Compton B., Anderson R.J., Painter G.F., Weinkove R., F Hermans I, & Berridge M.V. (2020). Vaccines adjuvanted with an NKT cell agonist induce effective T-cell responses in models of CNS lymphoma. Immunotherapy, 12(6).

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