Lncap

Manufactured by Atlanta Biologicals

The LNCaP is a cell line derived from a human prostate cancer metastatic site. It is commonly used in research applications involving prostate cancer and androgen receptor studies.

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4 protocols using lncap

Prostate Cell Line Cultivation and Authentication

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Non-malignant epithelial prostate cell lines (RWPE-1 and PWR-1E) and prostate carcinoma cell lines (LNCaP, Du145, PC3) were obtained from the American Type Culture Collection (ATCC) and cultured under recommended conditions as described previously (28 (link)). RWPE-1 and PWR-1E cells were cultured in keratinocyte growth medium supplemented with 5 ng/mL human recombinant epidermal growth factor, 0.05 mg/mL bovine pituitary extract (Invitrogen). LNCaP, Du145, PC3 were maintained in RPMI 1640 media supplemented with 10% fetal bovine serum (FBS) (Atlanta biologicals) and 1% penicillin/streptomycin. Cell lines were maintained in an incubator with a humidified atmosphere of 95% air and 5% CO2 at 37°C.
Cell lines were authenticated by DNA short-tandem repeat analysis by ATCC. The experiments with cell lines were performed within 6 months of their procurement/resuscitation.

Saini S., Majid S., Shahryari V., Tabatabai Z.L., Arora S., Yamamura S., Tanaka Y., Dahiya R, & Deng G. (2014). Regulation of SRC kinases by microRNA-3607 located in a frequently deleted locus in prostate cancer. Molecular cancer therapeutics, 13(7), 1952-1963.

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Culturing Human Prostate Cancer Cell Lines

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Human prostate cancer cell lines LNCaP, DU145 and PC3 were obtained from American Type Culture Collection (ATCC, Rockville, MD). LNCaP cells were cultured in RPMI-1640 supplemented with 5% fetal bovine serum (Atlanta Biologicals, Atlanta GA) and antibiotics (pen/strep, Normocin and Gentamycin). DU145 and PC3 cell were cultured in Ham’s F12 (Meditech, VA) supplemented with 5% bovine calf serum (Atlanta Biologicals) and antibiotics (pen/strep, Normocin and Gentamycin). All cells were cultured at 37°C and 5% CO2. DU145 cells over-expressing full length human ID4 (DU145+ID4) is described elsewhere^{49 (link),50}.

Sharma P., Chinaranagari S, & Chaudhary J. (2015). Inhibitor of Differentiation 4 (ID4) Acts as an Inhibitor of ID-1, -2 and -3 and Promotes basic Helix Loop Helix (bHLH) E47 DNA Binding and Transcriptional Activity. Biochimie, 112, 139-150.

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Prostate Cancer Cell Line Culture

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All prostate cancer cell lines (CWR22Rv1, LNCaP, PC3, and VCaP) were purchased from ATCC (Manassas, VA). Cell culture reagents were obtained from Gibco/Thermo Fisher Scientific (Gaithersburgh, MD), unless otherwise specified. The 22Rv1 and LNCaP cell lines were maintained in phenol red-free RPMI 1640 Medium supplemented with 10% fetal bovine serum (FBS, Atlanta Biologicals, Flowery Branch, GA), 100 units/mL penicillin, 100 units/mL streptomycin, and 0.25 μg/mL amphotericin B. For all experiments involving addition of steroid hormone, cells were plated in maintenance media, then medium was replaced with RPMI Medium 1640 supplemented with 10% charcoal stripped FBS (all other media components remained unchanged) for approximately 7 h before treatment. PC3 cells were cultured in F-12K Nutrient Mixture medium (Gibco), and VCaP cells were maintained and treated in DMEM Medium (Gibco) supplemented with 10% FBS, 100 units/mL penicillin, 100 units/mL streptomycin, and 0.25 μg/mL amphotericin B.

Barbier R.H., McCrea E.M., Lee K.Y., Strope J.D., Risdon E.N., Price D.K., Chau C.H, & Figg W.D. (2021). Abiraterone induces SLCO1B3 expression in prostate cancer via microRNA-579-3p. Scientific Reports, 11, 10765.

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Prostate and Cervical Cancer Cell Lines

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LNCaP (clone FGC, CRL-2876), JEG-3 (HTB-36), 22RV1 (CRL-2505), PC-3 (CRL-1435), and DU145 (HTB-81) cells were purchased from American Type Culture Collection (ATCC). C4–2B^{41 (link)} cells were provided by Drs. Ruoxiang Wu and Leland Chung (Cedars-Sinai). BHPrS-1,, NHPrE-1, and BHPrE-1 cells were provided by Dr. Simon Hayward (NorthShore Research Institute)^{42 (link),43}. BHPrS-1 cells were maintained in RPMI supplemented with 5% FBS and NHPrE-1 and BHPrE-1 cells in F12/DMEM 1:1 medium containing 5% FBS, 1% Insulin-Transferrin-Selenium supplement, 10 ng/ml epidermal growth factor, 0.4% bovine pituitary extract and 1% antibiotic-antimycotic mix (all from Gibco). LNCaP, C4–2B, and 22RV1 were cultured in 10% premium fetal bovine serum (FBS, Atlanta Biologicals S11150) in RPMI 1640 + L-glutamine (Gibco). JEG-3 and DU145 cells were cultured in 10% FBS in MEM + Earle’s salts and L-Glutamine (Gibco). PC-3 cells were cultured in 10% FBS in F-12K Kaighn’s modification + L-Glutamine (Gibco). Cell culture experiments were performed in FBS-containing media.

Nanda J.S., Awadallah W.N., Kohrt S.E., Popovics P., Cates J.M., Mirosevich J., Clark P.E., Giannico G.A, & Grabowska M.M. (2020). Increased nuclear factor I/B expression in prostate cancer correlates with AR expression. The Prostate, 80(13), 1058-1070.

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