Hp5973 ms

The chemical components of E. globulus EO were determined using gas chromatography coupled to mass spectrometry (GC/MS) analysis conditions as described by Mekkaoui et al. [27 (link)]. Briefly, a Hewlett-Packard (HP6890) GC instrument coupled with an HP5973 MS and equipped with a 5% phenylmethyl silicone HP−5 MS capillary column (30 m × 0.25 mm × film thickness 0.25 μm) was used in GC analysis. The column temperature was increased from 50 °C for 5 min to 200 °C with a 4 °C/min rate. Helium with a 1.5 mL/min flow rate and split mode (flow: 112 mL/min, ratio: 1/74.7) was the carrier gas used. The hold time was 48 min, while the temperature of the injector and detector was 250 °C.
The machine was led by the computer system ″HP ChemStation″, managing the functioning of the machine and allowing us to follow the evolution of chromatographic analyses. Diluted samples (1/20 in methanol) of 1 μL were injected manually. In addition, 70 eV ionization voltage, 230 °C ion source temperature, and a 35–450 (m/z) scanning range were the MS operating conditions. Finally, the qualitative quantification of the different compounds was based on the percent area of each peak of the sample compounds and was confirmed by reference to their MS identities (Library of NIST/EPA/NIH MASS SPECTRAL LIBRARY Version 2.0, build 1 July 2002).

The chemical components of PGEO were determined using GC-MS analysis. Indeed, a Hewlett-Packard (HP6890) GC instrument (Santa Clara, CA, USA) coupled with an HP5973 MS and equipped with a 5% phenylmethyl silicone HP-5MS capillary column (30 m × 0.25 mm × film thickness 0.25 μm) was used in GC analysis. The used column temperature increased from 50 °C for 5 min to 200 °C with a 4 °C/min rate. Helium with a 1.5 mL/min flow rate and a split mode (flow: 112 mL/min, ratio: 1/74.7) was the used carrier gas. The hold time was 48 min, while the temperature of the injector and detector was 250 °C. The machine was led by a computer system type “HP ChemStation”, managing the functioning of the machine and allowing us to follow the evolution of chromatographic analyses. Diluted samples (1/20 in methanol) of 1 μL were injected manually. In addition, 70 eV ionization voltage, 230 °C ion source temperature, and 35–450 (m/z) scanning range were the MS operating conditions. Finally, the identification of different compounds was performed by the comparison of MS spectra with the library and matching the Kovats index (Library of NIST/EPA/NIH MASS SPECTRAL LIBRARY Version 2.0, build 1 July 2002). The quantification of the different compounds was obtained by internal normalization on the total area of peaks detected in each chromatogram.