Cells were suspended in hypotonic buffer (0.1 mM piperazine-N,N’-bis(2-ethane sulfonic acid) (PIPES) pH 6.5, 5 mM CaCl2, 5 mM dithiothreitol (DTT), 0.5% Triton X-100, 0.5% NP-40), and centrifuged at 1000× g. Cell pellets were sonicated in extraction buffer (0.35 M NaCl, 20 mM Tris-HCl pH 7.2, 12 mM MgCl2, 5 mM ethylene glycol bis(2-aminoethyl ether)tetraacetic acid (EGTA), 5 mM DTT) for 1 min three times on ice. The nuclear extract was incubated with HMGB1 antibody (Proteintech) and protein A/G agarose (Santa Cruz) for 3 h at 4 °C. Precipitates were collected by centrifugation and washed five times with lysis buffer. The DNA concentration (A260) of the precipitate solution was measured using a spectrophotometer (Beckman Coulter Life Sciences, Indianapolis, IN, USA).
Hmgb1 antibody
Manufactured by Proteintech
Sourced in China
The HMGB1 antibody is a laboratory reagent used to detect the High Mobility Group Box 1 (HMGB1) protein in various biological samples. HMGB1 is a highly conserved nuclear protein involved in the regulation of gene transcription and DNA repair. This antibody can be used for techniques such as Western blotting, immunohistochemistry, and ELISA to analyze the expression and localization of HMGB1 in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Protein a g agarose, by Santa Cruz Biotechnology (1 mentions)
Ir 780 iodide, by Merck Group (1 mentions)
Fitc conjugated secondary antibody, by Proteintech (1 mentions)
Cell counting kit 8 cck 8 calcein am, by Dojindo Laboratories (1 mentions)
Propidium iodide, by Dojindo Laboratories (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Doxorubicin hydrochloride dox hcl, by Meilun (1 mentions)
2 protocols using hmgb1 antibody
1
HMGB1 Chromatin Immunoprecipitation
2
PLGA-PEG Nanoparticles for Doxorubicin Delivery
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PLGA (MW:20000, 50:50)-PEG (MW: 2000) was purchased from Xi’an Ruixi Biotechnology Co., Ltd (Xi’an, China). IR780 iodide and Poly (vinyl alcohol) (PVA) were obtained from Sigma-Aldrich (USA). Doxorubicin hydrochloride (DOX·HCl) was produced by Dalian Meilun Biotechnology Co., Ltd (Dalian, China). Dichloromethane (CH2Cl2) and isopropanol were purchased from Chongqing Chuandong Chemical Co., Ltd (Chongqing, China). Cell Counting Kit-8 (CCK-8), Calcein AM, and propidium iodide (PI) were purchased from Dojindo (Japan). HMGB1 antibody, CRT antibody, and FITC-conjugated secondary antibody were obtained from Proteintech Group, Inc. (Wuhan, China). ATP-enhanced kit was obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China). HMGB1, IFN-γ, and TNF-α ELISA Kits were purchased from JingMei Biotechnology Co., Ltd (Shenzhen, China). All chemical agents were used without further purification.
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