The largest database of trusted experimental protocols

Alere filariasis test strip

Manufactured by Abbott
Sourced in United States

The Alere™ Filariasis Test Strip (FTS) is a rapid diagnostic test designed to detect the presence of filarial antigens in human blood. The test strip provides a qualitative result, indicating whether the sample is positive or negative for filarial infection.

Automatically generated - may contain errors

5 protocols using alere filariasis test strip

1

Community Survey for Filariasis Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols
In June-July 2014, adults and children ≥ 5 years of age were invited to participate in the community survey. Participants who signed a written consent form (assent plus written consent from at least one parent was required for children < 18 years of age) were screened for CFA using the Alere Filariasis Test Strip (FTS, Scarborough, ME, USA) [11 (link)]. Capillary blood was collected by finger prick using a sterile disposable lancet in a hematocrit tube. The blood (70 μl) was then transferred on the sample application pad of the strip following the manufacturer’s instructions. A single trained person read all of the tests at 10 min, and results were recorded as negative or positive. Participants with a positive FTS result were re-sampled between 10:00 PM and midnight to prepare two thick blood smears (70 µl) for detection of microfilariae (mf). Slides were dehaemoglobinized, stained with Giemsa within 24 h, and read by two independent microscopists. The arithmetic mean count from the two slides was recorded as mf density (expressed in mf/70 μl). All individuals with a negative FTS result were considered to be amicrofilaraemic.
+ Open protocol
+ Expand
2

Filarial Antigen Detection in Blood

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Binax Now Filariasis ICT (Alere Inc., Scarborough, ME) was used to detect the filarial antigen circulating in the blood stream in the baseline and pre-TAS studies. Briefly, capillary blood was collected from survey participants by finger prick using special sterile lancets and lancet holders. A graduated capillary tube having a capacity to hold 100 μl of blood was used to take blood from participants and blood was dispensed onto the test card immediately. For TAS, the Alere Filariasis Test Strip (FTS, Alere Inc., Scarborough, ME) was used. In this case, 75 μl of finger pricked whole blood was collected from the survey participants and applied to the test strip by capillary tube. The test results were interpreted strictly following the manufacturer’s instructions. Positive control filarial antigen was used to confirm the quality of the ICT and FTS as per provided instructions.
+ Open protocol
+ Expand
3

Rapid Screening for Circulating Filarial Antigen

Check if the same lab product or an alternative is used in the 5 most similar protocols
A total of 590 participants were screened for circulating filarial antigen (CFA). Seventy-five micro liters (75μl) of blood sample was taken by finger-pricking, then slowly added onto Alere Filariasis Test Strip (FTS) (Alere©, Waltham, United States), following the manufacturer’s instructions. Briefly, the test cards were placed on a table and left for 15 minutes before reading the results. For CFA positive samples, a second 75μl blood sample obtained by finger-prick was collected for a confirmatory test as described above.
+ Open protocol
+ Expand
4

Filarial Testing of Children in Ethiopia

Check if the same lab product or an alternative is used in the 5 most similar protocols
Children aged 6 or 7 years whose parents had given consent and who had themselves given assent, were tested using filarial test strips (the Alere Filariasis Test Strip [FTS], formerly Alere, Waltham, MA; now Abbott, Scarborough, ME) for LF and gave blood samples on Whatman paper number 2 for OV impact assessment. Fixed volume (75-μL) micropipettes were used to transfer whole blood samples obtained via fingerstick to the test strips. Test results were interpreted after 10 minutes according to the manufacturer’s instructions. From the same puncture, ∼100 μL of blood was placed on Whatman number 2 filter paper and set aside to dry. These dried blood spots (DBS) were packed in plastic bags with desiccant, placed in coolers, and transported to the OV molecular laboratory at the Ethiopian Public Health Institute (EPHI) in Addis Ababa. Dried blood spots were stored in refrigerators until analyzed, at which time the serum from the DBS was eluted and tested using the Onchocerciasis Elimination Program for the Americas Ov16 ELISA methodology.14 (link)
+ Open protocol
+ Expand
5

Rapid Diagnostic Screening for Filariasis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Individuals were prescreened in their home villages for W. bancrofti infection with a rapid diagnostic test that detects circulating filarial antigenemia (the Alere Filariasis Test Strip, FTS, Alere, Inc, Waltham, MA, USA) [8 (link)]. Persons with positive FTS results had blood collected between 21:30 and 23:00 for Mf testing by membrane filtration with 1 mL of anticoagulated venous blood (5μM, Nuclepore Corp., Pleasanton, CA, USA). Two microscopists independently read Giemsa-stained filters to assess Mf load.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!