Scl 10avp hplc

Manufactured by Shimadzu

Sourced in Japan

The SCL-10Avp is a high-performance liquid chromatography (HPLC) system controller module manufactured by Shimadzu. It is designed to control and manage the operation of HPLC instruments, including the pump, autosampler, and detector.

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Lab products found in correlation

Topspin, by Bruker (2 mentions) Microtof q high resolution ms, by Bruker (1 mentions) Spd m10a vp diode array detector, by Shimadzu (1 mentions) Kinetex column, by Phenomenex (1 mentions) Microtof q, by Bruker (1 mentions)

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SCL-10Avp (44 citations)

3 protocols using scl 10avp hplc

Analytical Characterization of Purified Compounds

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Analytical HPLC analysis were carried out with a SCL-10Avp HPLC with an SPD-M10Avp diode array detector (Shimadzu) using a reversed-phase column (Phenomenex, Kinetex, 2.6 μm, 4.6 × 100 mm). MS analysis were carried out with either a low resolution MS with a HPLC system (Agilent 1260 Infinity 6120 Quadropole LC/MS) with similar conditions and column as the analytical HPLC or a MicrOTOF-Q high resolution MS with direct injection (Bruker Daltonics).
For NMR analysis, the purified compounds were dried and dissolved in DMSO-d6 or MeOD (Eurisotop) (1) or a mixture of DMSO-d6 and CDCl₃ (2). The NMR spectra were recorded with a 500 MHz Bruker AVANCE-III NMR-system equipped with a liquid nitrogen cooled Prodigy BBO (CryoProbe) or a 600 MHz Bruker AVANCE-III NMR-system equipped with a liquid nitrogen cooled Prodigy TCI (inverted CryoProbe) at 298 K. The signals were internally referenced to the solvent signals or tetramethylsilane (TMS). The experiments included 1D spectral analysis (¹H,¹³C) and 2D measurements (COSY, HMBC, and HSQCDE). Topspin (Bruker Biospin) was used for spectral analysis.

Matroodi S., Siitonen V., Baral B., Yamada K., Akhgari A, & Metsä-Ketelä M. (2020). Genotyping-Guided Discovery of Persiamycin A From Sponge-Associated Halophilic Streptomonospora sp. PA3. Frontiers in Microbiology, 11, 1237.

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Fluorous Immobilization and Capture of Protein Complexes

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All fluorous immobilizations and captures were conducted at 25 °C. To immobilize fluorous-Vif_138-161 onto the fluorous silica, 125 nmols of the peptide (250 μL of 500 μM) were passed through the fluorous column using a Harvard Apparatus (Holliston, MA) syringe pump flowing at 15 μL/min. To test the specific binding of fluorous-Vif_138-161 to fluorous silica, the non-fluorous tagged Vif_135-158 peptide was incubated with a packed fluorous column and washed as described for fluorous-Vif_138-161. Unbound fluorous-Vif_138-161 was removed by washing with 800-1000 column volumes of 20% methanol using a Shimadzu SCL-10A VP HPLC, followed by 20 column volumes of wash buffer. To capture purified Elongin BC or Elongin BC from an E. coli lysate, 500 pmols of purified protein (100 μL of 5 μM) or 100 μLs of lysate were flowed through a fluorous-Vif_138-161 loaded column using a Harvard Apparatus (Holliston, MA) syringe pump flowing at 15 μL/min. The fluorous column was washed with 50 column volumes of wash buffer. Captured Elongin BC was eluted from the fluorous column by rapidly flowing 15 column volumes of quench buffer (0.8% formic acid, 0.8 M guanidine hydrochloride, pH 2.2) and mass analyzed as described above.

Marcsisin S.R., Liptak C., Marineau J., Bradner J.E, & Engen J.R. (2015). Tag and capture flow hydrogen exchange mass spectrometry with a fluorous-immobilized probe. Analytical chemistry, 87(12), 6349-6356.

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Comprehensive Compound Characterization by HPLC-MS and NMR

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The compounds were analysed by HPLC (SCL-10Avp HPLC with an SPD-M10Avp diode array detector, Shimadzu, Tokyo, Japan) using 15% MeOH with or without 0.1% formic acid to 100% MeOH using a KINETEX column (2.6u C18 100Å; 100 × 4.6 mm, Phenomenex, Torrance, CA, USA). Purified compounds were analysed by high resolution mass (MicrOTOF-Q, Bruker Daltonics, Bremen, Germany) by direct injection in positive ionization mode. For NMR analysis the purified and desiccated compounds were dissolved in MeOD or CDCl3. 1D measurements: ¹H and ¹³C NMR and 2D measurements (COSY, heteronuclear multiple bond correlation, HMBC, heteronuclear single quantum coherence HSQCDE, heteronuclear single quantum correlation, edited) were performed with following instruments: 600 MHz Bruker AVANCE-III NMR-system with a liquid nitrogen cooled Prodigy TCI (inverted CryoProbe) and a 500 MHz Bruker AVANCE-III NMR-system with a liquid nitrogen cooled Prodigy BBO (CryoProbe). The signals were internally referenced to tetramethylsilane. Topspin (Bruker Biospin) was used for spectral analysis.

Bikash B., Vilja S., Mitchell L., Keith Y., Mikael I., Mikko M.K, & Jarmo N. (2021). Differential regulation of undecylprodigiosin biosynthesis in the yeast-scavenging Streptomyces strain MBK6. FEMS Microbiology Letters, 368(8), fnab044.

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