Sb203580 p38 mapk

SNP and diethylamine (DEA) NONOate were purchased from Sigma Aldrich (MO, USA). The RBM3 antibody (ab134946) was from Abcam (Cambridge, MA, USA), and antibodies against, AMPKα (#5832), p-AMPKα (#2535), AKT (#9272), p-AKT (#4060), p-ERK1/2 (#4370), p38 (#9212), p-p38 (#4511), p-JNK1/2 (#4668), p-MKK3/MKK6 (#12280), cleaved PARP (#9541), and β-actin (#4970) were form Cell Signaling Technology (Beverly, MA, USA). Antibodies against ERK1 (sc-93), and JNK1 (sc-474) were from Santa Cruz Biotechnology (CA, USA). The 4,6-diamidino-2-phenylindole dihydrochloride (DAPI) was purchased from Sangon (Shanghai, China). Annexin V-FITC cell apoptosis kit was from Beyotime (Jiangsu, China). Inhibitors SB203580 (p38 MAPK) and SP600125 (JNK), were from Sigma Aldrich. Inhibitors LY294002 (AKT) and U0126 (MEK) were from Cell Signaling Technology. The siRNAs specific to human Rbm3, human miR-143 mimics and antisense inhibitor, and their negative control oligonucleotides were purchased from GenePharma (Shanghai, China).

Rotenone was obtained from Sigma-Aldrich Co., LLC (St. Louis, MO, USA), and PA (CAS no. 4852-22-6) was purchased from Yuan Ye (Shanghai, China). Antibodies against β-actin, cleaved caspase-9, cleaved caspase-3, cleaved PARP, phospho-ERK1/2, phospho-p38, p38, phospho-JNK1/2 were purchased from Cell Signaling Technology (Beverly, MA, USA), anti-ERK2 and JNK1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The 4, 6-diamidino-2-phenylindole dihydrochloride (DAPI) was purchased from Sangon (Shanghai, China). Inhibitors SB203580 (p38 MAPK) and SP600125 (JNK), were from Sigma Aldrich. Inhibitor U0126 (MEK) were from Cell Signaling Technology. The One-step TUNEL apoptosis assay kit was purchased from Beyotime (Shanghai, China). Fluorometric Intracellular ROS Kit was purchased from Sigma-Aldrich Co., LLC (St.Louis, MO, USA).