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Bx61 confocal laser scanning microscope

Manufactured by Olympus
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The BX61 confocal laser scanning microscope is a high-performance imaging system designed for advanced microscopy applications. It utilizes a laser source and a pinhole aperture to produce optical sections, enabling the acquisition of detailed, three-dimensional images of specimens. The BX61 offers high-resolution imaging capabilities and is suitable for a variety of research and analytical tasks.

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FV1000 BX61 confocal laser scanning microscope Confocal laser scanning microscope BX61 confocal microscope Laser scanning microscope Laser confocal microscope BX61 microscope Confocal microscope

2 protocols using bx61 confocal laser scanning microscope

1

Visualizing RNase Protein Localization

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The full‐length MdD1 and S1‐, S2‐, S3‐ and S9‐RNase cDNA sequences were cloned into vectors harbouring a yellow fluorescent protein (YFP) coding sequence to generate either N‐terminal or C‐terminal fusions (Meng et al., 2014; a). The PCR primers used are listed in Supplemental Table S2. The resulting constructs were transiently expressed in apple leaves as previously described (Meng et al., 2014). The YFP fluorescence was imaged 5 days after transformation using an Olympus BX61 confocal laser scanning microscope. The excitation wavelength for YFP fluorescence was 488 nm, and emission fluorescence was detected at 500–542 nm.
Gu Z., Li W., Doughty J., Meng D., Yang Q., Yuan H., Li Y., Chen Q., Yu J., Liu C.S, & Li T. (2019). A gamma‐thionin protein from apple, MdD1, is required for defence against S‐RNase‐induced inhibition of pollen tube prior to self/non‐self recognition. Plant Biotechnology Journal, 17(11), 2184-2198.
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2

Transient Expression of Fluorescent Fusion Proteins in Tobacco Leaves

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The PhCHS coding sequence was fused with the C-terminus of pCambia 1300-YFPC to generate a PhCHS-YFPC plasmid, and the sequence of PhRING-H2 was fused with the N-terminus of pCambia 1300-YFPN to form PhRING-H2-YFPN. The resulting constructs were transiently expressed in tobacco (Nicotiana benthamiana) leaves by Agrobacterium infiltration as previously described (Schütze et al., 2009 (link)). Yellow fluorescent protein (YFP) fluorescence was imaged after transformation for 72 h using an Olympus BX61 confocal laser scanning microscope. The excitation wavelength for YFP fluorescence was 488 nm, and emission fluorescence was detected at 500–542 nm. Primers used for the bimolecular fluorescence complementation (BiFC) assays are listed in Supplementary Table S1.
Gu Z., Men S., Zhu J., Hao Q., Tong N., Liu Z.A., Zhang H., Shu Q, & Wang L. (2019). Chalcone synthase is ubiquitinated and degraded via interactions with a RING-H2 protein in petals of Paeonia ‘He Xie’. Journal of Experimental Botany, 70(18), 4749-4762.
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