For histological analysis, mouse back skin was shaved, removed, fixed in 4% paraformaldehyde (MP; 150146) in 1X PBS, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H/E). For indirect immunofluorescence, mouse back skin was freshly frozen in OCT (Fisher; 4585), sectioned at 5 μm, and staining was performed using DAPI (Thermo Fisher; D1306: 1:1000) and the following primary antibodies: Ki67 (Cell Signaling, D3B5, 1:1000), K14 (chicken, 1:1000; rabbit, 1:1000; gift of Julie Segre, National Institutes of Health, Bethesda), Slug/Snai2 (Cell Signaling, C19G7, 1:1000), Fos (Santa Cruz Biotechnology, sc271243, 1:100), F4/80 (eBioscience, 14-4801-82, 1:200), anti-SMA (Abcam, ab5694, 1:500), Col17a1 (Abcam, ab184996, 1:200), or p63 (Santa Cruz Biotechnology, sc-8343, 1:50).
Ab184996
Manufactured by Santa Cruz Biotechnology
Ab184996 is a laboratory instrument designed for use in scientific research and analysis. It is a high-performance device that can be utilized for various experimental procedures. The core function of this product is to provide reliable and precise data, though its specific intended use is not included in this description.
Automatically generated - may contain errors
2 protocols using ab184996
1
Histological and Immunofluorescence Analysis of Mouse Skin
2
Histological and Immunofluorescence Analysis of Mouse Skin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For histological analysis, mouse back skin was shaved, removed, fixed in 4% paraformaldehyde (MP; 150146) in 1X PBS, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H/E). For indirect immunofluorescence, mouse back skin was freshly frozen in OCT (Fisher; 4585), sectioned at 5 μm, and staining was performed using DAPI (Thermo Fisher; D1306: 1:1000) and the following primary antibodies: Ki67 (Cell Signaling, D3B5, 1:1000), K14 (chicken, 1:1000; rabbit, 1:1000; gift of Julie Segre, National Institutes of Health, Bethesda), Slug/Snai2 (Cell Signaling, C19G7, 1:1000), Fos (Santa Cruz Biotechnology, sc271243, 1:100), F4/80 (eBioscience, 14-4801-82, 1:200), anti-SMA (Abcam, ab5694, 1:500), Col17a1 (Abcam, ab184996, 1:200), or p63 (Santa Cruz Biotechnology, sc-8343, 1:50).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!