Absolute pl quantum yield spectrometer c11347 quantaurus qy

The steady-state, delayed PL spectra and phosphorescence lifetime were measured on an Edinburgh FLSP 980 fluorescence spectrophotometer equipped with a xenon arc lamp (Xe900) and a microsecond flash-lamp (uF900). Absolute PL quantum yields were measured using a Hamamatsu absolute PL quantum yield spectrometer C11347 Quantaurus_QY. Delayed emission of NA/PCP (Δt = 1 s) was recorded on an Ocean Optics Spectrometer (QE Pro).

Solid film fluorescence quantum yields were measured, using a Hamamatsu absolute PL quantum yield spectrometer C11347 Quantaurus_QY (Hamamatsu City, Shizuoka Prefecture, Japan) and a matched integrating sphere. Data showed averaged values of three detected readings from the machine.

All the chemicals and reagents were purchased from a commercial source and used as received without further purification. ¹H and ¹³C NMR spectra were measured on a Bruker AV 500 (500 MHz) spectrometer in CD₂Cl₂ with tetramethylsilane (TMS, δ = 0). High-resolution mass spectra (HRMS) were recorded on a GCT premier CAB048 mass spectrometer operating in MALDI-TOF mode. Single-crystal X-ray diffraction intensity data were collected on a Bruker–Nonices Smart Apex CCD diffractometer with graphite monochromated MoKα radiation. Processing of the intensity data was carried out using the SAINT and SADABS routines, and the structure and refinement were conducted using the SHELTL suite of X-ray programs (version 6.10). UV–vis absorption spectrum was measured on a Shimadzu UV-2600 spectrophotometer. PL spectra were recorded on a Horiba Fluoromax-4 spectrofluorometer. Solution fluorescence quantum yields were measured using a Hamamatsu absolute PL quantum yield spectrometer C11347 Quantaurus_QY. High-performance liquid chromatography spectra were measured using Waters alliance e2695 separation module.

The organic solvents used for spectroscopy measurement were spectra grade, and the water was Milli-Q water (Millipore, Burlington, MA, USA). Sunflower oil, stabilized with about 0.02% of d-δ-tocopherol, was obtained from Wako. The UV-Vis absorption spectra were performed on a JASCO V-770 spectrophotometer (JASCO Group, Tokyo, Japan), and the fluorescence spectra and quantum yields were obtained using a Hamamatsu Photonics Quantaurus-QY Absolute PL quantum yield spectrometer C11347 (Hamamatsu Photonics, Yokohama, Japan). Dynamic light scattering (DLS) measurements were performed on a Zetasizer Nano ZS analyzer (Malvern Instruments Ltd., Malvern, UK).

All reagents were purchased from commercial sources (Innochem (Beijing, China), TCI (Shanghai, China), Sigma-Aldrich (Shanghai, China), Adamas-beta (Shanghai, China), Aladdin (Shanghai, China)) and used without further purification. All solvents and inorganic salts were purchased from Beijing Reagent, Sinopharm Chemical Reagent Beijing or General-Reagent, and used without further processing. Human cervical carcinoma cells (HeLa cells), human hepatocarcinoma cells (HepG-2 cells), human non-small cell lung cancer cell (A549 cells), and Human Umbilical Vein Endothelial Cells (HU-EVC cells) were purchased from the Center of Cells, Peking Union Medical College.
¹H NMR and ¹³C NMR spectra were obtained with Bruker Avance III 400 and Ascend 600 MHz spectrometers (Karlsruhe, Germany), respectively, using CDCl₃ or DMSO-d₆ as a solvent, and tetramethylsilane (TMS) was used as an internal standard. Electrospray Ionization Mass Spectrometry (ESI-MS) was conducted using a Thermo Fisher Scientific Q-Exactive spectrometer (Waltham, MA, USA). Absorption spectra were recorded on a Hitachi U-3900 ultraviolet–visible (UV-Vis) spectrophotometer (Tokyo, Japan). Fluorescence spectra were recorded on a Hitachi F-4600 spectrofluorometer (Tokyo, Japan). Absolute fluorescence quantum yields were measured in a Quantaurus-QY absolute PL quantum yield spectrometer C11347 (Hamamatsu, Shizuoka, Japan).