Data acquisition was performed as indicated using either a four-laser FACSAria II cell sorter (no UV laser), a FACSAria Fusion cell sorter, or a BD LSRII analytical cytometer (BD Biosciences) each equipped with five lasers: UV (355 nm), violet (405 nm), blue (488 nm), yellow-green (561 nm), and red (637 nm). Cytometer calibration and control fluorescent measurements were performed using SPHERO eight-peak Rainbow Calibration Particles (Spherotech, Lake Forest, IL). Compensation was calculated using FACS DIVA Software (BD Biosciences) acquisition-defined matrices. Data were analyzed with FlowJo 10 (TreeStar, Ashland, OR).
Sphero eight peak rainbow calibration particles
Manufactured by Spherotech
SPHERO eight-peak Rainbow Calibration Particles are a set of fluorescent microspheres designed for the calibration and optimization of flow cytometry instruments. Each set contains eight distinct populations of particles with different fluorescence intensities, allowing users to assess the performance and sensitivity of their flow cytometry system.
Automatically generated - may contain errors
2 protocols using sphero eight peak rainbow calibration particles
1
Multiparameter Flow Cytometry Workflow
2
Multiparameter Flow Cytometry Workflow
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Data acquisition was performed as indicated using either a four-laser FACSAria II cell sorter (no UV laser), a FACSAria Fusion cell sorter, or a BD LSRII analytical cytometer (BD Biosciences) each equipped with five lasers: UV (355 nm), violet (405 nm), blue (488 nm), yellow-green (561 nm), and red (637 nm). Cytometer calibration and control fluorescent measurements were performed using SPHERO eight-peak Rainbow Calibration Particles (Spherotech, Lake Forest, IL). Compensation was calculated using FACS DIVA Software (BD Biosciences) acquisition-defined matrices. Data were analyzed with FlowJo 10 (TreeStar, Ashland, OR).
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