Nano s instrument

Example 9

This example presents one DNA fish oil/milk embodiment without any added emulsifiers that maintains particle diameter for at least three (3) weeks. The step-wise procedure is as follows:

1. Heat 1.5 g DHA fish oil, 50° C.

2. Heat 200 mL whole milk, 50° C.

3. Mix the two together

4. Stir and heat, 10 mins

5. Microfluidize using a M-1 10EH unit once at 25,000 PSI

6. Do particle diameter analysis using a Malvern Nano S instrument

The mean particle diameter (i.e., Peak 1) for the DHA fish oil/milk microfluidized nanoemulsion 93 nm. This nano-emulsion preparation was made without any added emulsifiers. Three weeks after the microfluidization process, the fish oil was still in solution and the particle diameter was again determined and found not to have changed. See FIG. 9. The average particle diameter data from the three-week microfluidized sample is presented in Table 11.

Example 2

This example presents one cod liver oil embodiment of a microfluidized nanoemulsion that has a stable particle diameter for at least four months. The step-wise procedure is as follows:

1. Heat 5 g of soybean oil (65° C.)

2. Add 5 g cod liver oil, stir and heat to 80° C.

3. Add 6 g polysorbate 80, stir and heat 20 mins

4. Add 200 mL de-ionized water, stir and heat 30 mins

5. Microfluidize using a M-1 10EH unit once at 25,000 PSI

6. Do particle diameter analysis using a Malvern Nano S instrument

The mean particle diameter (i.e., Peak I/Peak 2) for this cod liver oil microfluidized nanoemulsion was 58 nm. Before microfluidization, the mean particle diameter of the cod liver oil suspension was 2,842 nm. This represents a 50-fold reduction with a single pass through the microfluidizer. Four months after the microfluidization process, the particle diameter was again determined and found not to have changed. See FIG. 2. The average particle diameter data from the four-month microfluidized sample is presented in Table 4.