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Colorimetric creatinine assay kit

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The Colorimetric Creatinine Assay Kit is a laboratory tool used to quantitatively measure the concentration of creatinine in biological samples, such as serum, plasma, urine, and other fluids. The kit utilizes a colorimetric reaction to determine the creatinine levels, providing a reliable and efficient method for researchers and scientists to assess creatinine levels in their experiments and analyses.

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Colorimetric assay kit (281 citations) Colorimetric assay (66 citations) Creatinine Assay Kit (47 citations) Creatinine (11 citations) Creatinine Colorimetric/Fluorometric Assay Kit (10 citations)

2 protocols using colorimetric creatinine assay kit

1

Urinary Biomarkers in Preterm Infants

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We obtained 1.5 mL of spot urine samples from the preterm infants in the morning on days of life (DOL) 7, 14, 21, and 28; the samples were stored at –80°C until use. After thawing at 20–26°C, the samples were centrifuged at 300 × g for 10 min at 4°C. Urinary 8-OHdG and NT-proBNP levels were measured using enzyme-linked immunosorbent assay kits (8-OHdG: cat#: CEA660Ge, Uscn Life Science Inc., Wuhan, P.R. China; NT-proBNP: cat#: SEA485Hu, Uscn Life Science Inc., Wuhan, P.R. China). We also measured urine creatinine (Cr) by spectrophotometry and a colorimetric creatinine assay kit (cat#: ab204537, Abcam, Cambridge, United Kingdom) to correct diuresis variability from fluid restriction and the application of caffeine and diuretics. The results were expressed as ng of 8-OHdG per mg of Cr [i.e., the urinary 8-OHdG/creatinine ratio (UDGCR), ng/mg Cr] and ng of NT-proBNP per mg of Cr [i.e., the urinary NT-proBNP/creatinine ratio (UNBCR), ng/mg Cr]. All samples were analyzed in duplicate.
Cui X, & Fu J. (2022). Urinary biomarkers for the early prediction of bronchopulmonary dysplasia in preterm infants: A pilot study. Frontiers in Pediatrics, 10, 959513.
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2

Colorimetric Creatinine Quantification

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Concentration of creatinine was measured using Colorimetric Creatinine Assay Kit (Abcam, Cambridge, UK) according to the manufacturer’s instructions.
Needham B.D., Funabashi M., Adame M.D., Wang Z., Boktor J.C., Haney J., Wu W.L., Rabut C., Ladinsky M.S., Hwang S.J., Guo Y., Zhu Q., Griffiths J.A., Knight R., Bjorkman P.J., Shapiro M.G., Geschwind D.H., Holschneider D.P., Fischbach M.A, & Mazmanian S.K. (2022). A gut-derived metabolite alters brain activity and anxiety behaviour in mice. Nature, 602(7898), 647-653.
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