Tc 510

Manufactured by Millar

Sourced in United States

The TC-510 is a laboratory centrifuge designed for general-purpose applications. It features a brushless motor and a digital display for speed and time control. The TC-510 can accommodate a variety of sample tubes and microplates.

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Lab products found in correlation

6 f pigtail catheter, by Cordis (1 mentions) Bridge amp, by ADInstruments (1 mentions) Powerlab 4sp, by ADInstruments (1 mentions) Spr 320, by Millar (1 mentions)

Spelling variants of this product

TC-510 control unit (2 citations)

3 protocols using tc 510

Left Ventricular Pressure-Volume Measurement

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Our method for high fidelity, simultaneous LV pressure and volume recording has been described previously (Chung and Kovács 2008; Shmuylovich and Kovács 2008; Ghosh and Kovács 2012). In brief, simultaneous LV pressure and volume signals were acquired using a 6‐F triple transducer pigtail‐tipped P‐V conductance catheter (SSD‐1034; Millar Instruments, Houston, TX). Signals were calibrated using standard transducer control units (TC‐510; Millar Instruments). The aortic valve was crossed using fluoroscopy and the catheter was advanced toward the left ventricular apex, taking care to find a position that did not generate ectopic beats to assure stable hemodynamic recording. Pressure and volume signals were fed into clinical monitoring systems (Quinton Diagnostics, Bothell, WA or GE Healthcare, Milwaukee, WI) and a custom personal computer via a research interface (Sigma‐5DF; CD Leycom, Zoetermeer, The Netherlands) at a sampling rate of 250 Hz. LV ejection fraction was calculated from a calibrated ventriculogram (33 mL of contrast at 11 mL/sec through a 6‐F pigtail catheter (Cordis Corporation, NJ)) whose end‐systolic and end‐diastolic volumes were used to calibrate the volume channel.

Kohli K, & Kovács S.J. (2017). The quest for load‐independent left ventricular chamber properties: exploring the normalized pressure–volume loop. Physiological Reports, 5(6), e13160.

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Ventricular Pressure Measurement and Dobutamine Challenge

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Under the anesthesia of urethane (1 g/kg, i.p.), a catheter specially designed to measure ventricular pressure (SPR 320: Millar Instruments, Houston, TX, USA) was inserted into the LV via the right carotid. The catheter was connected, through a dedicated signal coupler (TC-510, Millar Instruments, USA), to a recording system (Bridge Amp attached to PowerLab/4SP, AD Instruments, Sydney, Australia) and, under continuous recording of LV pressure, they received increasing doses of dobutamine (1, 3, 10 and 15 μg/kg) intravenously. Dobutamine was used as a pharmacological stressor, and each dose was injected, in bolus, with an interval of at least 10 min between each other. The 1^st derivative in time of the LV pressure (dP/dt) was calculated online, and the maximum rate of increasing pressure was used as an index of systolic function of the rats.

Omoto A.C., Gava F.N., Silva C.A., Silva H.B., Parente J.M., Costa R.M., Castro M.M., Tostes R.D., Salgado H.C, & Fazan R J.r. (2018). Lack of scarring is not always a sign of cardiac health: Functional and molecular characterization of the rat heart’s following chronic reperfusion. PLoS ONE, 13(12), e0209190.

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Invasive Hemodynamic Monitoring in Rats

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On the day of the final experimental protocol, rats were anesthetized initially with a 5% isoflurane-oxygen mixture and maintained on a 3% isoflurane-oxygen mixture. A midline incision was made on the anterior portion of the neck and the right carotid artery was isolated, exteriorized, and cannulated with a 2-Fr-catheter-tipped pressure micromanometer (model TC-510, Millar Instruments). Heart rate (HR) and the arterial pressure were measured and then the micromanometer was advanced in a retrograde manner into the left ventricle (LV) and LV end-diastolic pressure (LVEDP) and the derivative of the pressure wave form (LV dP/dt) were measured. Following the LVEDP and LV dP/dt measurements, the mircomanometer was retracted and removed and replaced by a catheter (PE-10 connected to PE-50, IntraMedic polyethylene tubing, Clay Adams, Becton, Dickinson, Sparks, MD) that was placed in the ascending aorta. This catheter was used for the measurements of MAP and HR (model 200, DigiMed BPA, Louisville, KY) and the infusion of radiolabeled microspheres. A second catheter was placed in the caudal (tail) artery for arterial blood sampling (Musch & Terrell, 1992 ). Both catheters were tunneled subcutaneously to the dorsal aspect of the neck, exteriorized, anesthesia was terminated, and the rats were given >60 min to recover (Flaim et al, 1984 (link)).

Smith J.R., Ferguson S.K., Hageman K.S., Harms C.A., Poole D.C, & Musch T.I. (2017). Dietary Nitrate Supplementation Opposes the Elevated Diaphragm Blood Flow in Chronic Heart Failure during Submaximal Exercise. Respiratory physiology & neurobiology, 247, 140-145.

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