Chemical reagents for preparing buffers and the BCA Assay Kit were purchased from Sigma Aldrich, U.K. and Fisher Scientific, U.K. Buffers used include RIPA buffer, phosphate-buffered saline (PBS), Tris-buffered saline (TBS), blocking buffer, cell lysis buffer, elution buffer, SDS sample buffer, and ECL detection reagent [23 ]. PolyPlus INTERFERin was purchased from Source Bioscience, U.K. Pre-designed siRNA, Opti-MEM, Power SYBR Green, RNA to cDNA kit, and gel casting materials were purchased from Life Technologies, U.K. Antibodies, Protein A/G agarose gel beads, and protein ladders were obtained from Santa Cruz Biotechnology, UK and Cell Signalling Technologies, U.K. Recombinant mouse proteins, ELISA antibodies and detection reagents were purchased from Peprotech Ltd. DMEM culture medium and other cell culture materials were purchased from Lonza, U.K. PCR primers were designed with Primer3 Plus Bioinformatics Software and NCBI BLAST and purchased from Eurofins Genomics.
Protein ladders
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom
Protein ladders are molecular weight standards used in protein electrophoresis to determine the approximate molecular weight of proteins. They contain a mixture of proteins of known molecular weights, which serve as reference points for comparison with the sample proteins.
Automatically generated - may contain errors
Lab products found in correlation
Power sybr green, by Thermo Fisher Scientific (2 mentions)
Bca assay kit, by Merck Group (2 mentions)
Pre designed sirna, by Thermo Fisher Scientific (2 mentions)
Polyplus interferin, by Source Bioscience (2 mentions)
Protein a g agarose gel beads, by Santa Cruz Biotechnology (2 mentions)
Rna to cdna kit, by Thermo Fisher Scientific (2 mentions)
Dmem culture medium, by Lonza (2 mentions)
Opti mem, by Thermo Fisher Scientific (2 mentions)
Ncbi blast, by Eurofins (1 mentions)
2 protocols using protein ladders
1
Biochemical Assays and Cell Culture Techniques
2
Protein expression and gene silencing protocol
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Chemicals reagents used to prepare buffers and BCA Assay kit were purchased from Sigma Aldrich, UK and Fisher Scientific, UK. Buffers used include RIPA buffer, Phosphate-buffered saline (PBS), Tris-buffered saline (TBS), Blocking Buffer, Cell lysis buffer, elution buffer, SDS sample buffer, and ECL detection reagent [23] . PolyPlus INTERFERin was purchased from Source Bioscience, UK. Pre-designed siRNA, Opti-MEM, Power SYBR Green, RNa to cDNA kit, and gel casting materials were purchased from Life Technologies, UK.
Antibodies, Protein A/G agarose gel beads, and protein ladders were obtained from Santa Cruz Biotechnology, UK and Cell Signalling Technologies, UK. DMEM culture medium and other cell culture materials were purchased from Lonza, UK. PCR primers were designed with Primer3 Plus Bioinformatics Softwaew and NCBI BLAST, and purchased from Eurofins Genomics. The cells were incubated at 37ºC, 5% CO2 with Opti-MEM for 24 hours for efficient gene silencing.
Antibodies, Protein A/G agarose gel beads, and protein ladders were obtained from Santa Cruz Biotechnology, UK and Cell Signalling Technologies, UK. DMEM culture medium and other cell culture materials were purchased from Lonza, UK. PCR primers were designed with Primer3 Plus Bioinformatics Softwaew and NCBI BLAST, and purchased from Eurofins Genomics. The cells were incubated at 37ºC, 5% CO2 with Opti-MEM for 24 hours for efficient gene silencing.
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