Shzfas1 2

Plasmid extraction kit was obtained from Sangon Biotech (Shanghai, China). The short hairpin RNA (shRNA) for silencing ZFAS1 (shZFAS1#1, shZFAS1#2) and the negative control (shNC) were purchased from Genepharma (Shanghai, China). The pcDNA-ZFAS1, pcDNA-DDX21 and blank vector (NC) were synthesized by Genewiz (Suzhou, China). All of the shRNA nucleotide sequences were listed in Supplementary Tables 3, 4. Cells were cultured on 6-well plates to 60%-70% density and then transfected by using Lipofectamine 2000 (Invitrogen, USA) according to the manufacturer’s instructions. The cells were used for further experiments after 48 hours of transfection.

The short hairpin RNA (shRNA) for silencing ZFAS1 (shZFAS1#1, shZFAS1#2) and the negative control (shNC) were obtained from Genepharma (Shanghai, China). The pcDNA-ZFAS1, pcDNA-IMP2, shIMP2#1, shIMP2#2 and blank vector (NC) were purchased by Genechem (Shanghai, China). All of the shRNA nucleotide sequences were listed in Additional file 1: Tables S10–S11. Plasmid extraction kit was purchased from Sangon Biotech (Shanghai, China). The cells were cultured on a small dish to a density of 60–70%, and then transfected with Lipofectamine 8000 (Invitrogen, USA) according to the manufacturer's instructions. Cells were collected 48 h after transfection and used for further experiments.