When the MOI was 0.5, the morphology of DCs in different groups was observed under an optical microscope at 24 h. p. i. A positive control consisting of 100 ng/mL lipopolysaccharide (LPS)-stimulated DCs was included. LPS was obtained from Sigma-Aldrich (Missouri, USA). DCs were randomly selected and their lengths measured. The morphology index was calculated from 30 DCs randomly selected from five separate experiments, with six cells from each experiment (morphological index = longest axis/shortest axis) [40 (link)]. To determine endocytosis, cells were incubated with 1 mg/mL FITC-dextran (Sigma-Aldrich, Missouri, USA) with LPS as a positive control at 37℃ for 30 min [40 (link)] and detected via FACS. Phenotypes, activation, and migration of DCs were determined by FACS after incubating the cells with various antibodies, including PE-CD80, FITC-CD86, PE-CD40, FITC-MHC II, PerCP-Cy5.5-CD69, and PE-CCR7 (BD Biosciences, NY, USA).
Percp cy5.5 cd69
Manufactured by BD
Sourced in United States
PerCP-Cy5.5-CD69 is a fluorescent-conjugated antibody used in flow cytometry applications to detect and analyze the expression of the CD69 antigen on the surface of cells. It consists of the PerCP-Cy5.5 fluorophore coupled to an antibody specific for the CD69 protein.
Automatically generated - may contain errors
Lab products found in correlation
Fitc dextran, by Merck Group (2 mentions)
Cd80 pe, by BD (2 mentions)
Cd86 fitc, by BD (2 mentions)
Ccr7 pe, by BD (2 mentions)
Cd40 pe, by BD (2 mentions)
Fitc mhc 2, by BD (2 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Cck 8 kit, by Beyotime (1 mentions)
Dextran alexa fluor 647, by Thermo Fisher Scientific (1 mentions)
Cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions)
Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions)
Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions)
Gm csf, by Thermo Fisher Scientific (1 mentions)
Astaxanthin, by Merck Group (1 mentions)
Recombinant ccl19, by Thermo Fisher Scientific (1 mentions)
Cobalt protoporphyrin, by Merck Group (1 mentions)
Tin protoporphyrin 9, by MedChemExpress (1 mentions)
2 protocols using percp cy5.5 cd69
1
Morphological and Functional Characterization of DCs
2
Astaxanthin Modulates Macrophage Immune Responses
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Astaxanthin (mol wt 596.84), LPS derived from Escherichia coli 026: B6, FITC-Dextran (mol wt 40,000) and Cobalt protoporphyrin (CoPP, a HO-1 inducer) were from Sigma-Aldrich. Alexa Fluor 647-Dextran (mol wt 10,000) was from Thermo Fisher. Carboxyfluorescein succinimidylester (CFSE) and RPMI 1640 medium were from Invitrogen. Fetal bovine serum (FBS) was from Hyclone. Recombinant CCL19, GM-CSF, and IL-4 were from Peprotech. CCK-8 kit was from Beyotime. CD4+ T cell isolation kit was from Miltenyi Biotech. Fluorescent-labeled anti-mouse mAbs, PerCP-Cy5.5 CD69, FITC-MHCII, PE-CD40, PE-CD80, FITC-CD86, PE-CCR7 or respective isotype controls, were from BD PharMingen. Alexa Fluor 647 HO-1 or respective isotype was from Abcam. PE-Nrf2 or respective isotype was from Cell Signaling Technology. Tin protoporphyrin IX (SnPP, a HO-1 inhibitor) was from MedChemExpress.
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