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Bdgaspak ez pouch system

Manufactured by Coy Laboratory Products
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The BDGasPak™ EZ pouch system is a lab equipment product designed for the anaerobic cultivation of microorganisms. It provides an anaerobic environment for the growth of anaerobic bacteria and other anaerobic microorganisms.

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Lab products found in correlation

Phenotype microarray, by Biolog (2 mentions) Anaerobic chamber, by Coy Laboratory Products (2 mentions)

2 protocols using bdgaspak ez pouch system

1

Characterizing L. monocytogenes Growth on Xylitol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All L. monocytogenes strains used in this study were
derived from wildtype 10403S (Supplementary Table 4). Transduction methods were used to introduce
transposons into distinct genetic backgrounds, as previously
described.37 (link),38 (link)L. monocytogenes cells were grown at 37 °C and
spectrophotometrically measured by optical density at a wavelength of 600 nm
(OD600). Anaerobic conditions were achieved with the BD
GasPak™ EZ pouch system or an anaerobic chamber (Coy Laboratory Products)
with an environment of 2% H2 balanced in N2.
Filter-sterilized brain-heart infusion medium (Difco) or variants of
chemically defined Listeria synthetic medium (LSM)39 (link) were used in all studies.
“Aerobic respiration medium” replaced the glucose in LSM with 50
mM glycerol. The requirement of an electron acceptor to support L.
monocytogenes growth on xylitol was identified by comparing aerobic
versus anaerobic (absent an alternative electron acceptor) growth on carbon
sources, using PM1 and PM2A plates of the Phenotype MicroArray (Biolog).
“Xylitol medium” replaced the glucose in LSM with 50 mM
xylitol.
Light S.H., Su L., Rivera-Lugo R., Cornejo J.A., Louie A., Iavarone A.T., Ajo-Franklin C.M, & Portnoy D.A. (2018). A flavin-based extracellular electron transfer mechanism in diverse gram-positive bacteria. Nature, 562(7725), 140-144.
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2

Characterizing L. monocytogenes Growth on Xylitol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All L. monocytogenes strains used in this study were
derived from wildtype 10403S (Supplementary Table 4). Transduction methods were used to introduce
transposons into distinct genetic backgrounds, as previously
described.37 (link),38 (link)L. monocytogenes cells were grown at 37 °C and
spectrophotometrically measured by optical density at a wavelength of 600 nm
(OD600). Anaerobic conditions were achieved with the BD
GasPak™ EZ pouch system or an anaerobic chamber (Coy Laboratory Products)
with an environment of 2% H2 balanced in N2.
Filter-sterilized brain-heart infusion medium (Difco) or variants of
chemically defined Listeria synthetic medium (LSM)39 (link) were used in all studies.
“Aerobic respiration medium” replaced the glucose in LSM with 50
mM glycerol. The requirement of an electron acceptor to support L.
monocytogenes growth on xylitol was identified by comparing aerobic
versus anaerobic (absent an alternative electron acceptor) growth on carbon
sources, using PM1 and PM2A plates of the Phenotype MicroArray (Biolog).
“Xylitol medium” replaced the glucose in LSM with 50 mM
xylitol.
Light S.H., Su L., Rivera-Lugo R., Cornejo J.A., Louie A., Iavarone A.T., Ajo-Franklin C.M, & Portnoy D.A. (2018). A flavin-based extracellular electron transfer mechanism in diverse gram-positive bacteria. Nature, 562(7725), 140-144.
+ Open protocol
+ Expand

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