Plasma FN was from Corning (Bedford, MA, USA, #356008) Alexa Fluor 488- or 647-conjugated phalloidin and Hoechst 33342 were purchased from Invitrogen (Eugene, Oregon, USA, #A12379, #A22287, #H3570). Reagents for the 3D sprouting angiogenesis assay (Fibrinogen/#F8630, Thrombin/#T4648, Aprotinin/#A1153) were purchased from Sigma-Aldrich (Saint-Louis, MI, USA). Antibodies and oligonucleotides used in this study are indicated in the Key Resources Table (Appendix 1 ). All raw data associated with this manuscript are available in the source data files. All materials generated in this study are available upon request.
Alexa fluor 488 or 647 conjugated phalloidin
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alexa Fluor 488- or 647-conjugated phalloidin is a fluorescently labeled phallotoxin used for the detection and visualization of F-actin in cells. It binds specifically to F-actin and can be detected using fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using alexa fluor 488 or 647 conjugated phalloidin
1
3D Sprouting Angiogenesis Assay
2
Fluorescent Labeling of Hair Cells
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Fixed and permeabilized specimens underwent incubation with Alexa Fluor 488- or 647-conjugated phalloidin (1:1000; Invitrogen) for 30 min away from light and DAPI counterstaining for 10 min, for visualizing hair cell F-actin and nuclei, respectively.
3
Multimodal Analysis of Lamins and Cytoskeleton
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The following antibodies, isotypic IgGs and fluorescent dyes were used: monoclonal mouse anti-lamin A/C IgG1 (clone 131C3; Acris), polyclonal goat anti-lamin A/C (sc6215; Santa Cruz Biotechnology), polyclonal goat anti-lamin B1 (sc6217-M20; Santa Cruz Biotechnology), monoclonal mouse anti-lamin B2 IgG (clone X223; Progen), polyclonal rabbit anti-lamin B2 (10895-1-AP; ProteinTechGroup); monoclonal mouse anti-β-tubulin IgG1 (clone E7; Developmental Studies Hybridoma Bank, University of Iowa); monoclonal rabbit anti-GAPDH IgG (clone 14C10; Cell Signaling Technology) and polyclonal anti-βactin (Abcam) were used for confocal microscopy or western blotting. Secondary goat antimouse IgG and rabbit anti-goat IgG conjugated to horseradish peroxide (ECL; Thermo Fisher Scientific), or a fluorophore specific to the Odyssee system (IRDye 680RD and IRDye 800RD; LI-COR Biosciences) were used for western blotting, and secondary Alexa Fluor 488-conjugated pre-absorbed goat anti-mouse, donkey anti-goat and goat anti-rabbit IgGs (Invitrogen), Alexa Fluor 488 or 647-conjugated phalloidin (Invitrogen), and DAPI (Sigma) were used for confocal microscopy.
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