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2 protocols using polyethylene glycol peg 400

1

Formulation and Characterization of Lipid-Based Drug Delivery Systems

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Capmul MCM (HLB 4.7) was obtained as a gift from Abitec Corporation (Columbus, OH). The composition of Capmul MCM is approximately 60% of medium-chain monoglycerides and 40% of diglycerides, derived from caprylic acid (83%) and capric acid (17%). Danazol was donated by Sanofi-Aventis Pharmaceutics (Bridgewater, NJ). Caprylic acid (HLB 1), indomethacin, haloperidol, hydrochloric acid (1 N), potassium chloride, potassium phosphate monobasic, sodium hydroxide, sodium acetate, acetic acid, and polyethylene glycol (PEG) 400 were purchased from VWR (Solon, OH). Cremophor RH40 (HLB 14–16) was supplied by BASF Corporation (Tarrytown, NJ). All chemicals and solvents were of analytical purity or high performance liquid chromatography (HPLC) grade and used as received with no further treatment.
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2

Synthesis of Manganese Ferrite Nanoparticles

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The manganese ferrite nanoparticles were prepared from the following ingredients: manganese (II) nitrate tetrahydrate / Mn(NO3)2 ∙ 4 H2O (Carl Roth GmbH, Karlsruhe, Germany), iron (III) nitrate nonahydrate / Fe(NO3)3 ∙ 9 H2O (VWR International, Leuven, Belgium), and polyethylene glycol (PEG 400, [MW = 400 g/mol]) from VWR International (Fontenay-sous-Bois, France) and they were used as a reducing agent and dispersion media for the metal precursors.
For the maintenance of bacterial cell cultures and isolation of plasmid DNA with MnFe2O4 nanoparticles the following chemicals were used: tryptone, yeast extract (Neogen Culture Media, Lansing, MI, USA), sodium chloride, bacteriological agar, polyethylene glycol (VWR International, Leuven, Belgium), ampicillin sodium salt (Alfa Aesar, Kandel, Germany), a plasmid purification midi kit (Qiagen, Hilden, Germany), tris hydrochloride salt, bromophenol blue sodium salt (VWR International, Solon, OH, USA), ethylenediaminetetraacetic acid disodium salt dihydrate (Sigma-Aldrich, Louis, MO, USA), 96% ethanol (VWR International, Fontenay-sous-Bois, France), Tween 20, Gel Red nucleic acid gel stain, agarose (Merck Millipore, Billerica, MA, USA), and a 1 kb DNA ladder (New England Biolabs, Ipswich, MA, USA and Thermo Fisher Scientific, Waltham, MA, USA).
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