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2 protocols using ulture media

1

Culturing and Expanding Human MSCs

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Culture media, trypsin and antibiotics used for cell cultures were purchased from Euroclone (Milan, Italy). Collagenase, mannitol, bovine serum albumin, Nile Red, acetone, phosphatidylcholine, DPPH, were obtained from Sigma-Aldrich (Milan, Italy). A commercial platelet lysate kit (PL) was obtained from Carlo Erba Reagents (Milan, Italy). Collagenase and FBS were suitable for the production of clinical-grade human MSCs.
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2

Adipocyte Differentiation Protocol

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Culture media, fetal bovine serum (FBS), penicillin, streptomycin, and L-glutamine were obtained from Euroclone (Milan, Italy). BPA, 17b estradiol (E 2 ), collagenase type 1, human insulin, recombinant human epidermal growth factor (rEGF), and oil red O (ORO) powder were purchased from Sigma-Aldrich. Trizol reagent and a Human Interleukin 1 Beta (IL1B) ELISA kit were obtained from Invitrogen Life Technologies. High-Capacity cDNA Reverse Transcription kit was purchased from Applied Biosystems. A GeneChip Human Gene 1.0 ST array was obtained from Affymetrix (Santa Clara, CA, USA). IL18 and CCL20 ELISA kits and a Triglyceride Quantification kit were obtained from Abcam (Cambridge, UK). A human insulin ELISA kit was purchased from Millipore (St. Charles, MO, USA).
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