LTB4, IL-1β, IL-6, and TNF-α in the cell culture supernatants were measured using LTB4 EIA Kit (Cayman Chemicals) and Mouse IL-1β, IL-6, and TNF-α ELISA MAX Standard Kit (Biolegend), respectively, using manufacturer's instructions. Absorbance was measured using a BioTek microplate reader.
Mouse il 1β
Manufactured by BioLegend
Sourced in United States
Mouse IL-1β is a cytokine that plays a crucial role in the inflammatory response. It is involved in the regulation of immune responses, cell proliferation, and differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Agilent Technologies (1 mentions)
Tnf α elisa max standard kit, by BioLegend (1 mentions)
Ltb4 eia kit, by Cayman Chemical (1 mentions)
Caspase 1, by Novus Biologicals (1 mentions)
Mouse il 6, by BD (1 mentions)
Mouse tnf α, by BD (1 mentions)
Mouse il 10, by BioLegend (1 mentions)
Mouse il 4, by BioLegend (1 mentions)
Mouse il 6, by BioLegend (1 mentions)
3 protocols using mouse il 1β
1
Quantifying Inflammatory Mediators
2
Cytokine Quantification in Activated Cells
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Cells were seeded into 12‐well plates and treated with indicated activators and indicated bacteria. The supernatant was collected at the indicated time points and then quantified by using following commercially available ELISA kits: mouse IL‐1β (Biolegend, 432616), Caspase‐1 (Novus biologicals, NBP2‐75014), mouse TNF‐α (BD OptEIA, 555268), mouse IL‐6 (BD OptEIA, 555240), and mouse IL‐18 (MBL, 065FA).
3
Cytokine and Chemokine Profiling in Mouse Lungs
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The concentrations of human PK2 were determined by a human PK2 enzyme-linked immunosorbent assay kit (My BioSource, San Diego, CA, USA) according to the manufacturer's instructions.
For the detection of cytokines and chemokines in the mouse lung, the mice were euthanized after 6 and 24 hours of modeling, and then the lungs were excised and homogenized, then centrifuged (12,0 0 0 g × 10 minutes), and the supernatant was absorbed for the detection of cytokines and chemokines according to the manufacturer's instructions, including mouse IL-1 β (BioLegend, CA, USA), mouse tumor necrosis factor-α (BioLegend), mouse IL-17A (BioLegend), mouse IL-6 (BioLegend), mouse IL-10 (BioLegend), mouse IL-4 (BioLegend), mouse interferon-γ (BioLegend), mouse IL-27 p28/IL-30 (R&D Systems, MN, USA), mouse C-X-C ligand 1/KC (R&D Systems), and mouse C-C ligand 2/MCP-1 (R&D Systems).
For the detection of cytokines and chemokines in the mouse lung, the mice were euthanized after 6 and 24 hours of modeling, and then the lungs were excised and homogenized, then centrifuged (12,0 0 0 g × 10 minutes), and the supernatant was absorbed for the detection of cytokines and chemokines according to the manufacturer's instructions, including mouse IL-1 β (BioLegend, CA, USA), mouse tumor necrosis factor-α (BioLegend), mouse IL-17A (BioLegend), mouse IL-6 (BioLegend), mouse IL-10 (BioLegend), mouse IL-4 (BioLegend), mouse interferon-γ (BioLegend), mouse IL-27 p28/IL-30 (R&D Systems, MN, USA), mouse C-X-C ligand 1/KC (R&D Systems), and mouse C-C ligand 2/MCP-1 (R&D Systems).
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