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Human igg vector set

Manufactured by Merck Group
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The Human IgG Vector Set is a laboratory product designed for the expression and purification of human immunoglobulin G (IgG) antibodies. It provides the necessary tools and components to facilitate the production and isolation of human IgG in a research or experimental setting. The set includes the necessary vectors, cell lines, and protocols to enable the expression and purification of human IgG. The core function of this product is to support the study and manipulation of human IgG antibodies in a controlled laboratory environment.

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Lab products found in correlation

Expicho expression system, by Thermo Fisher Scientific (2 mentions) Expi293 expression system kit, by Thermo Fisher Scientific (2 mentions) Nab protein a plus spin column, by Thermo Fisher Scientific (2 mentions)

2 protocols using human igg vector set

1

Recombinant Antibody Expression Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Heavy and light chain sequences of BCRs of interest were synthesized as gene fragments (IDT, Gene Universal) and cloned into human IgG, IgK, and IgL expression vectors (Human IgG Vector Set, Sigma) according to the manufacturer’s instructions. Recombinant expression of antibodies was performed as previously described using the Expi293 Expression System Kit (Thermo Fisher Scientific) (3.63 (link)) or the ExpiCHO Expression System (Thermo Fisher Scientific). Filtered cell culture supernatant was used for dot blot and VirScan characterization. Antibody purification was performed using NAb Protein A Plus Spin Column (Thermo Fisher Scientific) according to the manufacturer’s instructions.
Shrock E.L., Timms R.T., Kula T., Mena E.L., West AP J.r., Guo R., Lee I.H., Cohen A.A., McKay L.G., Bi C., K.e.e.r.t.i., Leng Y., Fujimura E., Horns F., Li M., Wesemann D.R., Griffiths A., Gewurz B.E., Bjorkman P.J, & Elledge S.J. (2023). Germline-encoded amino acid-binding motifs drive immunodominant public antibody responses. Science (New York, N.Y.), 380(6640), eadc9498.
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2

Recombinant Antibody Expression Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Heavy and light chain sequences of BCRs of interest were synthesized as gene fragments (IDT, Gene Universal) and cloned into human IgG, IgK, and IgL expression vectors (Human IgG Vector Set, Sigma) according to the manufacturer’s instructions. Recombinant expression of antibodies was performed as previously described using the Expi293 Expression System Kit (Thermo Fisher Scientific) (3.63 (link)) or the ExpiCHO Expression System (Thermo Fisher Scientific). Filtered cell culture supernatant was used for dot blot and VirScan characterization. Antibody purification was performed using NAb Protein A Plus Spin Column (Thermo Fisher Scientific) according to the manufacturer’s instructions.
Shrock E.L., Timms R.T., Kula T., Mena E.L., West AP J.r., Guo R., Lee I.H., Cohen A.A., McKay L.G., Bi C., K.e.e.r.t.i., Leng Y., Fujimura E., Horns F., Li M., Wesemann D.R., Griffiths A., Gewurz B.E., Bjorkman P.J, & Elledge S.J. (2023). Germline-encoded amino acid-binding motifs drive immunodominant public antibody responses. Science (New York, N.Y.), 380(6640), eadc9498.
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