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Macsxpress cd4 t cell isolation kit

Manufactured by Miltenyi Biotec
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Sourced in United States

The MACSxpress CD4 T cell Isolation Kit is a lab equipment product designed for the isolation of CD4+ T cells from human peripheral blood mononuclear cells (PBMCs). The kit utilizes magnetic beads coated with antibodies specific for the CD4 surface marker to selectively bind and isolate the CD4+ T cell population.

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Lab products found in correlation

Rlt buffer, by Qiagen (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Allprep dna rna mini kit, by Qiagen (1 mentions) Phosphate buffered saline (pbs), by Biowest (1 mentions) Anti cd3 cd28 antibodies, by BD (1 mentions)

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Cell isolation kits Isolation kits

2 protocols using macsxpress cd4 t cell isolation kit

1

Isolation and Extraction of CD4+ T Cells

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CD4+ T cells were negatively isolated using the MACSxpress CD4 T cell Isolation Kit (Miltenyi). For non-CD4+ cell isolation, the column was removed from the magnet and washed with MACS buffer containing PBS (Biowest), EDTA (FLUKA), and BSA (Sigma) to release non-CD4+ cells. Cells were lysed in RLT+ buffer (Qiagen). DNA was extracted using AllPrep DNA/RNA Mini Kit (Qiagen).
Nissen S.K., Christiansen M., Helleberg M., Kjær K., Jørgensen S.E., Gerstoft J., Katzenstein T.L., Benfield T., Kronborg G., Larsen C.S., Laursen A., Pedersen G., Jakobsen M.R., Tolstrup M, & Mogensen T.H. (2018). Whole Exome Sequencing of HIV-1 long-term non-progressors identifies rare variants in genes encoding innate immune sensors and signaling molecules. Scientific Reports, 8, 15253.
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2

Isolation and Activation of CD4+ T Cells

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We isolated CD4+ T cells from human blood obtained from healthy volunteers, using MACSxpress CD4 T cell isolation kit (Miltenyi Biotec, Auburn, CA, USA). Briefly, whole blood was mixed with cocktail solution, then cells were separated in the magnetic field. Supernatant was collected and centrifuged, then resuspended in red blood cell lysis buffer. After centrifugation, cells were suspended in medium and stimulated with anti-CD3/CD28 antibodies (BD Biosciences, Franklin Lakes, NJ, USA).
Li Z.J., Choi D.K., Sohn K.C., Lim S.K., Im M., Lee Y., Seo Y.J., Kim C.D, & Lee J.H. (2016). Induction of Interleukin-22 (IL-22) production in CD4+ T Cells by IL-17A Secreted from CpG-Stimulated Keratinocytes. Annals of Dermatology, 28(5), 579-585.
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