The plasmid coding for human EGFR tagged with EGFP (hEGFR-EGFP) was a kind gift from Alexander Sorkin (Addgene plasmid #32751) [27 (link)]. To replace EGFP with mEGFP, both plasmids hEGFR-EGFP and mEGFP-N1 (gift from Michael Davidson, Addgene plasmid #54767) were digested with AgeI and NotI (New England Biolabs GmbH, Ipswich, MA, USA). The hemagglutinin (HA) gene from influenza virus strain A/FPV/Rostock/34 (H7N1) tagged with mEGFP at the C-terminus (FPV-HA-mEGFP) was cloned based on the previously described FPV-HA-mEYFP (gift from Andreas Herrmann, Humboldt University Berlin) [28 (link)]. Briefly, FPV-HA-mEYFP was digested using BglII and SacII (New England Biolabs GmbH, Ipswich, MA, USA), and the obtained HA-insert was ligated into mEGFP-N1. This plasmid is available on Addgene (#127810).
Megfp n1
Manufactured by New England Biolabs
Sourced in United States
MEGFP-N1 is a mammalian expression vector that encodes the monomeric enhanced green fluorescent protein (mEGFP). It is designed for the expression and visualization of mEGFP-tagged proteins in mammalian cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using megfp n1
1
Plasmid Engineering for EGFR-EGFP
2
Generating EGFR-EGFP and HA-mEGFP Plasmids
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The plasmid coding for human EGFR tagged with EGFP (hEGFR-EGFP) was a kind gift from Alexander Sorkin (Addgene plasmid #32751) (27) . To replace EGFP with mEGFP, both plasmids hEGFR-EGFP and mEGFP-N1 (gift from Michael Davidson, Addgene plasmid #54767) were digested with AgeI and NotI (New England Biolabs GmbH, Ipswich, MA). The hemagglutinin (HA) gene from influenza virus strain A/FPV/Rostock/34 (H7N1) tagged with mEGFP at the C-terminus (FPV-HA-mEGFP) was cloned based on the previously described FPV-HA-mEYFP (gift from Andreas Herrmann, Humboldt University Berlin) (28) . Briefly, FPV-HA-mEYFP was digested using BglII and SacII (New England Biolabs GmbH, Ipswich, MA), and the obtained HA-insert was ligated into mEGFP-N1. This plasmid is available on Addgene (#127810).
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