Vasopressin

Vasopressin was obtained from Cayman Chemical (Ann Arbor, MI). Angiotensin II and SII (Sar¹, Ile^4,8]-Angiotensin II)^{40 (link)} were obtained from Genscript (Piscataway, NJ) and MyBioSource (San Diego, CA), respectively. Trevena peptides TRV120026, TRV120045, and TRV120055^{47 (link)} were synthesized by Genscript. For clarity, the ligand names are abbreviated to TRV026, TRV045 and TRV055. All working concentration of drugs were dissolved in DPBS and added manually to the HEK 293 T cells at the indicated concentrations and time points.

The cDNA for the β₂ adrenergic, GLP-1, MOR, NOP, and V₂ vasopressin receptors, and the cDNA for GRK2, was obtained from the cDNA Resource Center (Bloomsburg University, Bloomsburg, PA). In some experiments receptors modified to include the C-terminal tail of the V₂ vasopressin receptor were used. This involved substituting the last C-terminal residues with the last 29 C-terminal residues of the V₂ receptor (Oakley et al., 2000 (link)) (last 72 amino acids of the β₂ adrenoceptor, and the last 29 amino acids of the MOR, NOP and GLP-1 receptors). DAMGO, met-enkephalin, endomorphin-2, morphine, hydromorphone, oxymorphone, fentanyl, buprenorphine, vasopressin, N/OFQ and exendin-4 were all obtained from Cayman Chemical (Ann Arbor, MI). Isoproterenol was from Millipore Sigma.